Biotechnology Letters ( IF 2.0 ) Pub Date : 2023-11-16 , DOI: 10.1007/s10529-023-03442-3 Jiancong Cao 1 , Bingrun Yang 1 , Mengxia Zhang 1 , Fang Yu 1
To investigate the effect of subcellular localization on the transformation efficiency of heterologous expressed functional P450s in yeast. Microbial biotransformation offers a promising substitute for the direct extraction of natural products, but its viability in industrial applications depends on achieving high transformation efficiencies. To investigate the influence of subcellular microenvironments on the activity of heterologously expressed P450s, Catharanthus roseus tabersonine 16-hydroxylase (T16H) was chosen, and its subcellular localization was regulated by fusing organelle-localization signals. Interestingly, this manipulation had no effect on the gene expression levels of T16H, but resulted in varying conversion rates from tabersonine to 16-hydroxy tabersonine. Notably, the highest transformation efficiency was observed in yeast cells expressing peroxisome-localized T16H. Given the alkaline pH optimum for P450s, the alkaline peroxisomal lumen could be a suitable compartment for P450s reactions to achieve high transformation efficiency using yeast cells. Different organelle-localization of T16H in yeast cells resulted in varying conversion rates, suggesting that compartmentalizing the expression of target enzymes could be a viable approach to increase transformation efficiency in yeast.
中文翻译:
调控T16H亚细胞定位以提高其在酵母细胞中的催化效率
目的 研究亚细胞定位对酵母中异源表达的功能性 P450 转化效率的影响。微生物生物转化为天然产物的直接提取提供了一种有前途的替代品,但其在工业应用中的可行性取决于实现高转化效率。为了研究亚细胞微环境对异源表达的P450活性的影响,选择了长春花tabersonine 16-羟化酶(T16H),并通过融合细胞器定位信号来调节其亚细胞定位。有趣的是,这种操作对T16H的基因表达水平没有影响,但导致从 tabersonine 到 16-羟基 tabersonine 的转化率不同。值得注意的是,在表达过氧化物酶体定位的T16H的酵母细胞中观察到最高的转化效率。鉴于 P450 的最佳碱性 pH 值,碱性过氧化物酶体腔可能是 P450 反应的合适隔室,以利用酵母细胞实现高转化效率。 T16H 在酵母细胞中的不同细胞器定位导致不同的转化率,这表明划分靶酶的表达可能是提高酵母转化效率的可行方法。