Analytical and Bioanalytical Chemistry ( IF 3.8 ) Pub Date : 2023-11-14 , DOI: 10.1007/s00216-023-05030-w Linlin Yu 1 , Yuting He 1 , Guofen Zhou 1 , Lianzhe Hu 1 , Min Wang 2
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Human urine phosphorus (existing in the form of phosphate) is a biomarker for the diagnosis of several diseases such as kidney disease, hyperthyroidism, and rickets. Therefore, the selective detection of phosphate in urine samples is crucial in the field of clinical diagnosis. Herein, we reported the phosphatase-like catalytic activity of few-layered h-BNNS for the first time. As the phosphatase-like activity of few-layered h-BNNS could be effectively inhibited by phosphate, a selective fluorescent method for the detection of phosphate was proposed. The linear range for phosphate detection is 0.5–10 µM with a detection limit of 0.33 µM. The fluorescent method was then explored for the detection of human urine phosphorus in real samples. The results obtained by the proposed method were consistent with those of the traditional method, indicating that the present method has potential application for urine phosphorus detection in clinical disease diagnosis.
Graphical Abstract
中文翻译：
少层氮化硼纳米片作为非金属磷酸酶纳米酶及其在人尿磷检测中的应用
人类尿磷（以磷酸盐的形式存在）是诊断多种疾病的生物标志物，例如肾脏疾病、甲状腺功能亢进症和佝偻病。因此，尿液样本中磷酸盐的选择性检测在临床诊断领域至关重要。在此，我们首次报道了少层 h-BNNS 的磷酸酶样催化活性。由于磷酸盐可以有效抑制少数层 h-BNNS 的磷酸酶样活性，因此提出了一种选择性荧光检测磷酸盐的方法。磷酸盐检测的线性范围为 0.5–10 μM，检测限为 0.33 μM。然后探索荧光方法检测实际样品中人尿磷。所提方法得到的结果与传统方法的结果一致，表明该方法在临床疾病诊断中具有潜在的尿磷检测应用。
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