5-Bromo-2'-脱氧尿苷标记：历史观点、影响检测的因素、毒性及其对神经发生的影响。,Neural Regeneration Research

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5-Bromo-2'-脱氧尿苷标记：历史观点、影响检测的因素、毒性及其对神经发生的影响。
Neural Regeneration Research ( IF 5.9 ) Pub Date : 2024-02-01 , DOI: 10.4103/1673-5374.379038
Joaquín Martí-Clúa ₁

Affiliation

卤代嘧啶 5-溴-2'-脱氧尿苷 (BrdU) 是 DNA 合成的外源标记。自从引入针对 BrdU 的单克隆抗体以来，越来越多的方法被用于免疫检测这种合成的溴标记碱基类似物进入复制 DNA。BrdU 标记广泛用于识别神经元前体并跟踪其在各种脊椎动物（包括鸟类、爬行动物和哺乳动物）的胚胎、围产期和成年神经发生过程中的命运。由于 BrdU 毒性，其掺入复制 DNA 会对细胞的生成、存活和固定模式产生不利影响。这可能会导致增殖神经母细胞鉴定中的错误结果和误解。在这篇综述中，我将指出这种核苷在中枢神经系统发育过程中的有害影响，以及 BrdU 标记检测增殖神经母细胞的可靠性。此外，还将展示影响 BrdU 免疫检测的因素以及该核苷对产前、围产期和成人神经发生研究的贡献。人类成人神经发生也将被讨论。我希望这篇综述能为那些专注于检测处于细胞周期合成阶段的细胞的研究人员提供参考。

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5-Bromo-2'-deoxyuridine labeling: historical perspectives, factors influencing the detection, toxicity, and its implications in the neurogenesis.

The halopyrimidine 5-bromo-2'-deoxyuridine (BrdU) is an exogenous marker of DNA synthesis. Since the introduction of monoclonal antibodies against BrdU, an increasing number of methodologies have been used for the immunodetection of this synthesized bromine-tagged base analogue into replicating DNA. BrdU labeling is widely used for identifying neuron precursors and following their fate during the embryonic, perinatal, and adult neurogenesis in a variety of vertebrate species including birds, reptiles, and mammals. Due to BrdU toxicity, its incorporation into replicating DNA presents adverse consequences on the generation, survival, and settled patterns of cells. This may lead to false results and misinterpretation in the identification of proliferative neuroblasts. In this review, I will indicate the detrimental effects of this nucleoside during the development of the central nervous system, as well as the reliability of BrdU labeling to detect proliferating neuroblasts. Moreover, it will show factors influencing BrdU immunodetection and the contribution of this nucleoside to the study of prenatal, perinatal, and adult neurogenesis. Human adult neurogenesis will also be discussed. It is my hope that this review serves as a reference for those researchers who focused on detecting cells that are in the synthetic phase of the cell cycle.

更新日期：2023-11-14

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