Development of heart organoid cryopreservation method through Fe3O4 nanoparticles based nanowarming system,Biotechnology Journal

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Development of heart organoid cryopreservation method through Fe3O4 nanoparticles based nanowarming system
Biotechnology Journal ( IF 3.2 ) Pub Date : 2023-11-12 , DOI: 10.1002/biot.202300311
Seul-Gi Lee ₁ , Jin Kim ₂ , Jin Seok ₂ , Min Woo Kim ₂ , Jooeon Rhee ₂ , Gyeong-Eun Song ₂ , Shinhye Park ₂ , Suemin Lee ₂ , Youngin Jeong ₂ , Hyung Min Chung _{1,

3} , C-Yoon Kim ₂

Affiliation

Beyond single cell two-dimensional (2D) culture, research on organoids that can mimic human organs is rapidly developing. However, there are still problems in commercialization and joint research using organoids due to the lack of technology to safely store organoids. Since organoids are 3D complex structures with a certain size (0.1–5 mm) beyond the size of cells, the conventional cell-level cryopreservation method using cryoprotectant (CPA) cannot overcome the damage caused by volume change due to osmotic pressure difference and ice nucleation. Herein, we attempted to solve such limitations by applying a nanowarming system using CPA with high cell permeability and Fe₃O₄ nanoparticles. By performing beat rate measurement, histological analysis, contractility analysis, and multi-electrode array, it was verified that the developed method could significantly improve functional recovery and survival of heart organoids after freezing and thawing. In this study, we demonstrated a successful organoid cryopreservation method based on a Fe₃O₄ nanowarming system. The developed technology will provide clues to the field of tissue cryopreservation and spur the application of organoids.

中文翻译：

基于 Fe3O4 纳米粒子的纳米加温系统开发心脏类器官冷冻保存方法

除了单细胞二维（2D）培养之外，模拟人体器官的类器官研究也在迅速发展。然而，由于缺乏安全储存类器官的技术，类器官的商业化和联合研究仍然存在问题。由于类器官是3D复杂结构，其尺寸（0.1-5毫米）超出了细胞的尺寸，传统的使用冷冻保护剂（CPA）的细胞水平冷冻保存方法无法克服由于渗透压差异和冰成核导致的体积变化所造成的损伤。在此，我们尝试通过应用使用具有高细胞渗透性的CPA和Fe ₃ O ₄纳米粒子的纳米升温系统来解决这些限制。通过进行搏动率测量、组织学分析、收缩性分析和多电极阵列，验证了所开发的方法可以显着提高心脏类器官冻融后的功能恢复和存活率。在这项研究中，我们展示了一种基于 Fe ₃ O ₄纳米加热系统的成功类器官冷冻保存方法。所开发的技术将为组织冷冻保存领域提供线索，并促进类器官的应用。

更新日期：2023-11-12

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