<br>具有局部神经生长因子释放的混合导电水凝胶促进周围神经再生,ACS Applied Bio Materials

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具有局部神经生长因子释放的混合导电水凝胶促进周围神经再生
ACS Applied Bio Materials ( IF 4.6 ) Pub Date : 2023-11-10 , DOI: 10.1021/acsabm.3c00977
Weijie Su ₁ , Jiakun Xu ₁ , Dating Pei _{2,

3,

4} , Xixi Li ₁ , Jia Yang ₁ , Zhijie Geng _{2,

3,

4} , Qunfeng Liu ₅ , Lixuan Yang ₁ , Shan Yu _{2,

3,

4}

Affiliation

周围神经损伤（PNI）的治疗在临床上具有挑战性。作为周围神经修复的金标准，自体神经移植仍然是一个关键的限制，包括组织可用性、供体部位发病率、免疫排斥等。近年来，导电水凝胶（CH）由于其良好的导电性、生物相容性和低免疫原性，在神经生物工程中显示出潜在的应用前景。在此，开发了一种由丙烯酸衍生物、明胶和肝素组成的具有持续神经生长因子（NGF）释放特性的混合导电水凝胶。使用大鼠坐骨神经损伤 (SNI) 模型（10 毫米长的节段缺损）来研究这些水凝胶导管促进周围神经修复的功效。结果表明，水凝胶导管具有优异的导电性、机械性能和生物相容性。此外，固定在水凝胶导管中的NGF具有良好的缓释特性。最后，功能恢复和电生理学评估以及组织学分析表明，固定NGF的水凝胶导管对运动恢复、轴突生长和髓鞘再生具有优异的效果，从而显着加速坐骨神经的修复。这项研究表明，具有局部 NGF 释放的混合导电水凝胶可有效用于 PNI 修复。

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Hybrid Electrically Conductive Hydrogels with Local Nerve Growth Factor Release Facilitate Peripheral Nerve Regeneration

It is challenging to treat peripheral nerve injury (PNI) clinically. As the gold standard for peripheral nerve repair, autologous nerve grafting remains a critical limitation, including tissue availability, donor-site morbidity, immune rejection, etc. Recently, conductive hydrogels (CHs) have shown potential applications in neural bioengineering due to their good conductivity, biocompatibility, and low immunogenicity. Herein, a hybrid electrically conductive hydrogel composed of acrylic acid derivatives, gelatin, and heparin with sustained nerve growth factor (NGF) release property was developed. The rat sciatic nerve injury (SNI) model (10 mm long segment defect) was used to investigate the efficacy of these hydrogel conduits in facilitating peripheral nerve repair. The results showed that the hydrogel conduits had excellent conductivity, mechanical properties, and biocompatibility. In addition, NGF immobilized in the hydrogel conduits had good sustained release characteristics. Finally, functional recovery and electrophysiological evaluations, together with histological analysis, indicated that the hydrogel conduits immobilizing NGF had superior effects on motor recovery, axon growth, and remyelination, thereby significantly accelerating the repairing of the sciatic nerve. This study demonstrated that hybrid electrically conductive hydrogels with local NGF release could be effectively used for PNI repair.

更新日期：2023-11-10

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