Zeolitic imidazole framework-67 (ZIF-67) was demonstrated to exhibit intrinsic peroxidase-like activity by using 3,3′,5,5′-tetramethylbenzidine (TMB) as a chromogenic substrate. However, due to the inherent defects in the chemical stability and thermal stability of ZIFs, the peroxidase-like activity of ZIF-67 was severely affected. Therefore, establishing a simple and effective method to enhance the stability of ZIF-67 is necessary. ATP was found to improve the catalytic activity of ZIF-67. On the one hand, compared with ZIF-67, the catalytic efficiency of ZIF-67/ATP increased by 796 %, 5010 % and 570 % at 25 °C, 30 °C and 40 °C, respectively. In addition, the catalytic activity of ZIF-67/ATP was also greatly enhanced at pH-4. On the other hand, compared with ATP, the catalytic rate of ZIF-67/ATP increased by 364 % after 10 min of incubation. Inspired by the high catalytic activity of ZIF-67/ATP, we used the ZIF-67/ATP peroxidase-like system for colorimetric detection of glutathione with the limit of detection (LOD) of 0.46 μM. Furthermore, the proposed method provides a perspective on stabilizing the structures of ZIFs and developing a simple and efficient detection method.

中文翻译：

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腺苷三磷酸酶稳定的 ZIF-67 作为过氧化物酶模拟物，用于快速检测谷胱甘肽

通过使用 3,3',5,5'-四甲基联苯胺 (TMB) 作为显色底物，证明沸石咪唑骨架 67 (ZIF-67) 表现出内在的过氧化物酶样活性。然而，由于ZIF的化学稳定性和热稳定性固有的缺陷，ZIF-67的类过氧化物酶活性受到严重影响。因此，有必要建立一种简单有效的方法来增强ZIF-67的稳定性。 ATP被发现可以提高ZIF-67的催化活性。一方面，与ZIF-67相比，ZIF-67/ATP的催化效率在25℃、30℃和40℃下分别提高了796%、5010%和570%。此外，ZIF-67/ATP的催化活性在pH-4下也大大增强。另一方面，与ATP相比，孵育10分钟后ZIF-67/ATP的催化率增加了364%。受ZIF-67/ATP高催化活性的启发，我们使用类ZIF-67/ATP过氧化物酶系统对谷胱甘肽进行比色检测，检测限（LOD）为0.46 μM。此外，所提出的方法为稳定 ZIF 结构和开发简单有效的检测方法提供了前景。