The current study pertains to the evaluation of the 2,4-di-tert-butyl phenol (2,4 DTBP) compound from the brown seaweed, Dictyota ciliolata against the MCF-7 breast cancer cell line. The 2,4-DTBP was confirmed by the spectral characterization of the HPLC, FTIR, ¹H NMR and ¹³C NMR. The DPPH antioxidant assay of the 2,4-DTBP revealed that the fractioned compound possesses higher antioxidant content of 59.02 % with the IC₅₀ value of 56.61 μL when compared to the standard ascorbic acid. Presently, the 2,4-DTBP had exhibited cytotoxicity as it induced the apoptosis process against MCF-7 with the IC₅₀ value of 70 μM. Molecular docking analysis showed that 2,4-DTBP exhibited high binding affinity with carbonic anhydrase IX enzyme with good docking score (−6.7 to −6.2 kcal/mol). The non-target, Artemia toxicity assay using 2,4-DTBP revealed only low toxicity rate with the LC₅₀ value (μg/mL) of 198.93, after 24 h treatment. The present study provides some insights into the medicinal and pharmaceutical properties of the 2,4-DTBP with special reference to the breast cancer cell-apoptosis. The presently generated baseline data would form important basis for further research on 2,4-DTBP towards anticancer drug formulation in future.

目前的研究涉及来自褐海藻Dictyota ciliolata的 2,4-二叔丁基苯酚 (2,4 DTBP) 化合物对MCF-7 乳腺癌细胞系的评估。2,4-DTBP 通过HPLC、FTIR、 ¹ H NMR 和¹³ C NMR的光谱表征得到证实。2,4-DTBP 的 DPPH 抗氧化剂测定表明，与标准抗坏血酸相比，分馏的化合物具有更高的抗氧化剂含量，为 59.02 %，IC _{50值为 56.61 μL。}目前，2,4-DTBP已表现出细胞毒性，它诱导针对MCF-7的细胞凋亡过程，IC ₅₀值为70 μM。分子对接分析表明，2,4-DTBP与碳酸酐酶IX表现出高结合亲和力，具有良好的对接分数（-6.7至-6.2 kcal/mol）。使用 2,4-DTBP 进行的非靶标卤虫毒性测定显示，处理 24 小时后，毒性率较低，LC _{50值 (μg/mL) 为 198.93。}本研究提供了对 2,4-DTBP 的医学和药物特性的一些见解，特别是乳腺癌细胞凋亡。目前生成的基线数据将为未来进一步研究 2,4-DTBP 抗癌药物制剂奠定重要基础。