Swab-in-Capillary Electrospray Ionization and a Miniature Mass Spectrometer for In Situ Drug Analysis,Analytical Chemistry

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Swab-in-Capillary Electrospray Ionization and a Miniature Mass Spectrometer for In Situ Drug Analysis
Analytical Chemistry ( IF 6.7 ) Pub Date : 2023-11-10 , DOI: 10.1021/acs.analchem.3c03279
Siyu Liu ₁ , Wei Xu ₁ , Yanbing Zhai ₁

Affiliation

In situ analysis of drugs has been in increasing demand in many fields. As an updated version of capillary-in-capillary electrospray ionization (CC-ESI) developed previously, a disposable swab-in-capillary electrospray ionization (SC-ESI) source was designed in this study. With a micro medical swab for sampling and an integrated filter membrane for online filtration, SC-ESI was able to directly sample and MS analyze complex samples without the need for pretreatment. Coupled with a miniature mass spectrometer, SC-ESI was applied for direct analysis of effective ingredients in therapeutic drugs (in tablet, capsule, and liquid droplet) and drugs in saliva and quantitation of therapeutic drugs in blood. The limits of detection in absolute amounts were obtained as 1 ng for fentanyl and 0.5 ng for cocaine in saliva. Combining with an internal standard method, SC-ESI acquired linear quantitation ranges of 100–5000 ng/mL for imatinib in whole blood and 100–2000 ng/mL for clozapine in serum with high accuracies and precisions. The entire analysis process, from sampling to data acquisition, can be completed in less than 2 min. As demonstrated as a cheap, portable, and sampling-effective ionization source, SC-ESI has shown great potential for in situ drug analysis, especially in border drug screening and clinical therapeutic drug monitoring.

中文翻译：

用于原位药物分析的毛细管拭子电喷雾电离和微型质谱仪

许多领域对药物原位分析的需求不断增加。作为之前开发的毛细管中毛细管电喷雾电离（CC-ESI）的更新版本，本研究设计了一次性拭子毛细管电喷雾电离（SC-ESI）源。 SC-ESI 借助微型医用拭子采样和集成滤膜在线过滤，能够直接对复杂样品进行采样和 MS 分析，无需预处理。 SC-ESI与微型质谱仪联用，可直接分析治疗药物（片剂、胶囊、液滴）和唾液中药物的有效成分以及血液中治疗药物的定量。唾液中芬太尼的绝对检测限为 1 ng，可卡因的绝对检测限为 0.5 ng。结合内标法，SC-ESI 获得了全血中伊马替尼 100-5000 ng/mL 和血清中氯氮平 100-2000 ng/mL 的线性定量范围，具有较高的准确度和精密度。整个分析过程，从采样到数据采集，可以在不到2分钟内完成。 SC-ESI 作为一种廉价、便携、采样有效的电离源，在原位药物分析方面显示出巨大的潜力，特别是在边界药物筛选和临床治疗药物监测方面。

更新日期：2023-11-10

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