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Improving Gibberellin GA3 Production with the Construction of a Genome-Scale Metabolic Model of Fusarium fujikuroi
Journal of Agricultural and Food Chemistry ( IF 5.7 ) Pub Date : 2023-11-06 , DOI: 10.1021/acs.jafc.3c05309
Ya-Wen Li 1 , Jin-Yi Qian 1 , Jia-Cong Huang 1 , Dong-Sheng Guo 1 , Zhi-Kui Nie 2 , Chao Ye 1 , Tian-Qiong Shi 1
Affiliation  

Liquid fermentation is the primary method for GA3 production usingFusarium fujikuroi. However, production capacity is limited due to unknown metabolic pathways. To address this, we constructed a genome-scale metabolic model (iCY1235) with 1753 reactions, 1979 metabolites, and 1235 genes to understand the GA3 regulation mechanisms. The model was validated by analyzing growth rates under different glucose uptake rates and identifying essential genes. We used the model to optimize fermentation conditions, including carbon sources and dissolved oxygen. Through the OptForce algorithm, we identified 20 reactions as targets. Overexpressing FFUJ_02053 and FFUJ_14337 resulted in a 37.5 and 75% increase in GA3 titers, respectively. These targets enhance carbon flux toward GA3 production. Our model holds promise for guiding the metabolic engineering of F. fujikuroi to achieve targeted overproduction. In summary, our study utilizes the iCY1235 model to understand GA3 regulation, optimize fermentation conditions, and identify specific targets for enhancing GA3 production through metabolic engineering.

中文翻译:


通过构建 Fujikuroi 镰刀菌基因组规模代谢模型提高赤霉素 GA3 产量



液体发酵是利用Fusarium fujikuroi生产GA 3的主要方法。然而,由于未知的代谢途径,生产能力受到限制。为了解决这个问题,我们构建了一个基因组规模的代谢模型( i CY1235),其中包含 1753 个反应、1979 个代谢物和 1235 个基因,以了解 GA 3调控机制。通过分析不同葡萄糖摄取率下的生长率并识别必需基因来验证该模型。我们使用该模型来优化发酵条件,包括碳源和溶解氧。通过 OptForce 算法,我们确定了 20 个反应作为目标。过表达 FFUJ_02053 和 FFUJ_14337 分别导致 GA3 滴度增加 37.5% 和 75%。这些目标提高了 GA 3生产的碳通量。我们的模型有望指导F. fujikuroi的代谢工程以实现目标过量生产。总之,我们的研究利用i CY1235 模型来了解 GA 3调节、优化发酵条件并确定通过代谢工程增强 GA 3产量的具体目标。
更新日期:2023-11-06
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