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One-pot synthesized copper-imidazole-2-carboxaldehyde complex material with oxidase-like activity for the colorimetric detection of glutathione and ascorbic acid
Heliyon ( IF 3.4 ) Pub Date : 2023-11-04 , DOI: 10.1016/j.heliyon.2023.e22099 Jia-Xin Li 1 , Jia-Li Wang 1 , Tong-Qing Chai 1 , Feng-Qing Yang 1
Heliyon ( IF 3.4 ) Pub Date : 2023-11-04 , DOI: 10.1016/j.heliyon.2023.e22099 Jia-Xin Li 1 , Jia-Li Wang 1 , Tong-Qing Chai 1 , Feng-Qing Yang 1
Affiliation
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Due to the copper (Cu) active sites, its complexes with oxidase-like activity have superior catalytic properties, which can catalyze a series of specific substrates like 3,3′,5,5′-tetramethylbenzidine (TMB), producing colorimetric reactions for the detection of different reducing small-molecule compounds. Attribute to the competitive coordination effects between water molecules and central Cu ions, most of the Cu complexes can hardly be used in the pure aqueous reaction system. In this study, a Cu-based material (Cu-imidazole-2-carboxaldehyde, Cu-ICA) was prepared using copper ions and ICA through a one-step process in the water solution. After the morphology of the material being characterized, the mimetic enzyme behavior of the Cu-ICA was demonstrated through the TMB oxidation. Compared to the other reported oxidase-like mimics, Cu-ICA has better aqueous stability and oxidase-like activity, and shows a higher . Furthermore, basing on the oxidase-like activity of Cu-ICA, a colorimetric method was developed for the ascorbic acid and glutathione detections with linear ranges of 0.5–5 μM and 0.5–4 μM, and limit of detection of 0.1304 μM and 0.097 μM, respectively. Owing to its excellent aqueous stability and oxidase-like activity, Cu-ICA has bright application prospects in the analysis of reducing small-molecule compounds.
中文翻译：
一锅合成具有类氧化酶活性的铜-咪唑-2-甲醛复合材料用于谷胱甘肽和抗坏血酸的比色检测
由于具有铜（Cu）活性位点，其具有类氧化酶活性的配合物具有优异的催化性能，可以催化3,3',5,5'-四甲基联苯胺（TMB）等一系列特定底物，产生比色反应不同还原性小分子化合物的检测。由于水分子与中心Cu离子之间的竞争配位效应，大多数Cu配合物很难用于纯水反应体系中。在这项研究中，使用铜离子和ICA通过一步法在水溶液中制备了一种铜基材料（Cu-imidazole-2-carbox醛，Cu-ICA）。在对材料的形态进行表征后，通过 TMB 氧化证明了 Cu-ICA 的模拟酶行为。与其他报道的类氧化酶模拟物相比，Cu-ICA 具有更好的水稳定性和类氧化酶活性，并表现出更高的活性。此外，基于 Cu-ICA 的类氧化酶活性，开发了一种用于检测抗坏血酸和谷胱甘肽的比色方法，线性范围为 0.5-5 μM 和 0.5-4 μM，检测限为 0.1304 μM 和 0.097 μM ， 分别。由于其优异的水稳定性和类氧化酶活性，Cu-ICA在还原性小分子化合物的分析中具有广阔的应用前景。
更新日期：2023-11-04
中文翻译：
一锅合成具有类氧化酶活性的铜-咪唑-2-甲醛复合材料用于谷胱甘肽和抗坏血酸的比色检测
由于具有铜（Cu）活性位点，其具有类氧化酶活性的配合物具有优异的催化性能，可以催化3,3',5,5'-四甲基联苯胺（TMB）等一系列特定底物，产生比色反应不同还原性小分子化合物的检测。由于水分子与中心Cu离子之间的竞争配位效应，大多数Cu配合物很难用于纯水反应体系中。在这项研究中，使用铜离子和ICA通过一步法在水溶液中制备了一种铜基材料（Cu-imidazole-2-carbox醛，Cu-ICA）。在对材料的形态进行表征后，通过 TMB 氧化证明了 Cu-ICA 的模拟酶行为。与其他报道的类氧化酶模拟物相比，Cu-ICA 具有更好的水稳定性和类氧化酶活性，并表现出更高的活性。此外，基于 Cu-ICA 的类氧化酶活性，开发了一种用于检测抗坏血酸和谷胱甘肽的比色方法，线性范围为 0.5-5 μM 和 0.5-4 μM，检测限为 0.1304 μM 和 0.097 μM ， 分别。由于其优异的水稳定性和类氧化酶活性，Cu-ICA在还原性小分子化合物的分析中具有广阔的应用前景。
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