乌龙茶萎凋过程中,非生物胁迫引起各种风味物质和茉莉酸(JA)含量的显着变化。然而,枯萎过程中染色质可及性的变化及其潜在影响仍然知之甚少。通过整合 ATAC-seq、RNA-seq、代谢物和激素测定,我们表征了枯萎处理引起的染色质可及性、基因表达水平、重要代谢物含量以及 JA 和 JA-ILE 含量的变化。此外,我们还分析了染色质可及性改变对基因表达变化、重要风味物质含量变化和 JA 超积累的影响。我们的分析确定了在萎凋处理下总共 3451 个开放和 13426 个封闭差异可及染色质区域 (DACR)。我们的研究结果表明,闭合DACRs介导的下调差异表达基因(DEG)导致萎凋过程中多种儿茶素的积累减少,而开放DACRs介导的上调差异表达基因(DEG)导致重要萜类化合物JA的积累增加, JA-ILE 和短链 C5/C6 挥发物。我们进一步强调了与儿茶素、萜类化合物、JA 和 JA 以及短链 C5/C6 挥发物合成相关的重要 DACR 介导的 DEG,并证实了近 DACR 对儿茶素合成的广泛影响,涉及萎凋过程中途径中的几乎所有酶。重要的是,我们鉴定了一种调节儿茶素合成的新型 MYB 转录因子 (CsMYB83),并使用 DAP-seq 验证了 CsMYB83 在关键儿茶素合成基因的启动子-DACRs 区域中的结合。 总体而言,我们的结果不仅揭示了乌龙茶萎凋过程中染色质改变介导的转录、风味物质和激素变化的情况,而且为茶树风味改良育种提供了靶基因。
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Chromatin accessibility mediated transcriptome changes contribute to flavor substance alterations and jasmonic acid hyperaccumulation during oolong tea withering process
During the oolong tea withering process, abiotic stresses induce significant changes in the content of various flavor substances and jasmonic acid (JA). However, the changes in chromatin accessibility during withering and their potential impact remain poorly understood. By integrating ATAC-seq, RNA-seq, metabolite, and hormone assays, we characterized the withering treatment-induced changes in chromatin accessibility, gene expression levels, important metabolite contents, and JA and JA-ILE contents. Additionally, we analyzed the effects of chromatin accessibility alterations on gene expression changes, content changes of important flavor substances, and JA hyperaccumulation. Our analysis identified a total of 3451 open- and 13 426 close-differentially accessible chromatin regions (DACRs) under withering treatment. Our findings indicate that close-DACRs-mediated down-regulated differentially expressed genes (DEGs) resulted in the reduced accumulation of multiple catechins during withering, whereas open-DACRs-mediated up-regulated DEGs contributed to the increased accumulation of important terpenoids, JA, JA-ILE and short-chain C5/C6 volatiles. We further highlighted important DACRs-mediated DEGs associated with the synthesis of catechins, terpenoids, JA and JA and short-chain C5/C6 volatiles and confirmed the broad effect of close-DACRs on catechin synthesis involving almost all enzymes in the pathway during withering. Importantly, we identified a novel MYB transcription factor (CsMYB83) regulating catechin synthesis and verified the binding of CsMYB83 in the promoter-DACRs regions of key catechin synthesis genes using DAP-seq. Overall, our results not only revealed a landscape of chromatin alters-mediated transcription, flavor substance and hormone changes under oolong tea withering, but also provided target genes for flavor improvement breeding in tea plant.