Substantial efforts are underway to deepen our understanding of human brain morphology, structure, and function using high-resolution imaging as well as high-content molecular profiling technologies. The current work adds to these approaches by providing a comprehensive and quantitative protein expression map of 13 anatomically distinct brain regions covering more than 11,000 proteins. This was enabled by the optimization, characterization, and implementation of a high-sensitivity and high-throughput microflow liquid chromatography timsTOF tandem mass spectrometry system (LC–MS/MS) capable of analyzing more than 2,000 consecutive samples prepared from formalin-fixed paraffin embedded (FFPE) material. Analysis of this proteomic resource highlighted brain region-enriched protein expression patterns and functional protein classes, protein localization differences between brain regions and individual markers for specific areas. To facilitate access to and ease further mining of the data by the scientific community, all data can be explored online in a purpose-built R Shiny app (https://brain-region-atlas.proteomics.ls.tum.de).

中文翻译：

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通过高通量蛋白质组学实现人脑区域分辨蛋白质组图


人们正在进行大量努力，利用高分辨率成像和高内涵分子分析技术来加深我们对人脑形态、结构和功能的理解。目前的工作通过提供涵盖 11,000 多种蛋白质的 13 个解剖学上不同的大脑区域的全面、定量的蛋白质表达图来补充这些方法。这是通过优化、表征和实施高灵敏度、高通量微流液相色谱 timsTOF 串联质谱系统 (LC-MS/MS) 实现的，该系统能够分析 2,000 多个由福尔马林固定石蜡包埋制备的连续样品（FFPE）材料。对这种蛋白质组资源的分析强调了大脑区域丰富的蛋白质表达模式和功能蛋白质类别、大脑区域之间的蛋白质定位差异以及特定区域的个体标记。为了方便科学界访问和进一步挖掘数据，所有数据都可以在专门构建的 R Shiny 应用程序 (https://brain-region-atlas.proteomics.ls.tum.de) 中在线探索。