第一个天然酵母菌株 Trichosporon asahii HZ10 具有强大的黄酮类化合物生产力及其潜在的生物合成途径,Journal of Agricultural and Food Chemistry

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第一个天然酵母菌株 Trichosporon asahii HZ10 具有强大的黄酮类化合物生产力及其潜在的生物合成途径
Journal of Agricultural and Food Chemistry ( IF 5.7 ) Pub Date : 2023-11-01 , DOI: 10.1021/acs.jafc.3c05188
Si-Jia Xue ₁ , Jie Liu ₁ , Xiao-Chen Li ₁ , Xin-Tong Zhang ₁ , Zhao-Zhe Xin ₁ , Wen-Wen Jiang ₁ , Jin-Yong Zhang _{1,

2}

Affiliation

黄酮类化合物通常被认为是重要的植物天然产物，具有多种生物活性和药理作用。通过植物提取和化学合成工业生产类黄酮的传统方法面临着严峻的经济和环境挑战。寻找满足绿色可持续发展的天然强效黄酮类化合物生产微生物是很好的选择之一。在这里，从原始蜂窝中分离出一种天然酵母Trichosporon asahii HZ10，发现其细胞内积累了 146.41 mg/L 的总黄酮。此外，T. asahii HZ10代表了广泛的黄酮代谢谱，涵盖了40种黄酮类化合物，其中近一半是水飞蓟宾、大豆黄酮和鸢尾素三甲醚，特别是水飞蓟宾占总黄酮的21.07%。这是世界上第一个产生类黄酮的天然酵母菌株。此外，T. asahii HZ10 衍生的黄酮类化合物具有良好的抗氧化活性。有趣的是，基因组挖掘和转录组分析清楚地表明，T. asahii HZ10可能在黄酮骨架合成的最关键步骤中进化出一条新的黄酮合成途径，这与植物和丝状真菌中的途径不同。因此，我们的研究结果不仅丰富了天然类黄酮生物合成途径的多样性，而且为促进微生物生产类黄酮的合成生物学策略的发展开辟了一条替代途径。

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First Natural Yeast Strain Trichosporon asahii HZ10 with Robust Flavonoid Productivity and Its Potential Biosynthetic Pathway

Flavonoids are generally thought to be essential plant natural products with diverse bioactivities and pharmacological effects. Conventional approaches for the industrial production of flavonoids through plant extraction and chemical synthesis face serious economic and environmental challenges. Searching for natural robust flavonoid-producing microorganisms satisfying green and sustainable development is one of the good alternatives. Here, a natural yeast, Trichosporon asahii HZ10, isolated from raw honeycombs, was found to accumulate 146.41 mg/L total flavonoids intracellularly. Also, T. asahii HZ10 represents a broad flavonoid metabolic profiling, covering 40 flavonoids, among which nearly half were silibinin, daidzein, and irigenin trimethyl ether, especially silibinin occupying 21.07% of the total flavonoids. This is the first flavonoid-producing natural yeast strain worldwide. Furthermore, T. asahii HZ10-derived flavonoids represent favorable antioxidant activities. Interestingly, genome mining and transcriptome analysis clearly showed that T. asahii HZ10 possibly evolves a novel flavonoid synthesis pathway for the most crucial step of flavonoid skeleton synthesis, which is different from that in plants and filamentous fungi. Therefore, our results not only enrich the diversity of the natural flavonoid biosynthesis pathway but also pave an alternative way to promote the development of a synthetic biology strategy for the microbial production of flavonoids.

更新日期：2023-11-01

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