<br>深入了解芽殖酵母减数分裂起始和定型转录回路中关键基因的识别和进化保护,FEBS Open Bio

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深入了解芽殖酵母减数分裂起始和定型转录回路中关键基因的识别和进化保护
FEBS Open Bio ( IF 2.8 ) Pub Date : 2023-10-31 , DOI: 10.1002/2211-5463.13728
Deepyaman Das _{1,

2} , Anis Ahmad Chaudhary ₃ , Mohamed A M Ali _{3,

4} , Abdullah S Alawam ₃ , Hironmoy Sarkar ₁ , Soumita Podder ₂

Affiliation

芽殖酵母减数分裂的启动并不使细胞进行减数分裂。因此，两种不同的信号级联可能会差异性地调节芽殖酵母的减数分裂起始和定型。为了区分这些信号级联的作用，我们重建了蛋白质-蛋白质相互作用网络和基因调控网络，其中基因在减数分裂起始和定型中上调。通过分析集成网络，我们确定了减数分裂起始的四个主调节因子 (MR) [Ume6p、Msn2p、Met31p、Ino2p]、三个转录因子 (TF) 和 279 个独特的靶基因 (TG)，以及三个 MR [Ndt80p、Aro80p、 Rds2p]、11 个 TF 和 948 个减数分裂特有的 TG。对减数分裂启动和定型转录级联中这些不同成员的功能富集分析表明，营养线索重新连接基因表达以启动减数分裂，而染色体重组使细胞进行减数分裂。由于减数分裂染色体重组在真核生物中高度保守，我们将酿酒酵母两个减数分裂阶段转录级联中独特成员的进化速率与子囊菌门成员进行了比较，揭示了减数分裂过程中控制染色体重组的转录级联经历了更大的进化速率。净化选择压力（ P值=0.0013、0.0382、0.0448、0.0369、0.02967、0.04937、0.03046、0.03357且<0。00001 分别为棉阿什比亚 (Ashbya gossypii) 、解脂耶氏酵母 (Yarrowia lipolytica) 、汉逊德巴利酵母 (Debaryomyces hansenii) 、烟曲霉 (Aspergillus fumigatus) 、粗糙脉孢菌 (Neurospora crassa) 、乳酸克鲁维酵母 (Kluyveromyceslactis)、粟酒裂殖酵母 ( Schizosaccharomyces pombe )、冷嗜裂殖酵母 (Schizosaccharomyces cyophilus ) 和八孢裂殖酵母 ( Schizosaccharomyces octosporus )。这项研究划分了驱动减数分裂启动和承诺的关键参与者，并证明了它们在芽殖酵母中的不同进化速率。

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Insights into the identification and evolutionary conservation of key genes in the transcriptional circuits of meiosis initiation and commitment in budding yeast

Initiation of meiosis in budding yeast does not commit the cells for meiosis. Thus, two distinct signaling cascades may differentially regulate meiosis initiation and commitment in budding yeast. To distinguish between the role of these signaling cascades, we reconstructed protein–protein interaction networks and gene regulatory networks with upregulated genes in meiosis initiation and commitment. Analyzing the integrated networks, we identified four master regulators (MRs) [Ume6p, Msn2p, Met31p, Ino2p], three transcription factors (TFs), and 279 target genes (TGs) unique for meiosis initiation, and three MRs [Ndt80p, Aro80p, Rds2p], 11 TFs, and 948 TGs unique for meiosis commitment. Functional enrichment analysis of these distinct members from the transcriptional cascades for meiosis initiation and commitment revealed that nutritional cues rewire gene expression for initiating meiosis and chromosomal recombination commits cells to meiosis. As meiotic chromosomal recombination is highly conserved in eukaryotes, we compared the evolutionary rate of unique members in the transcriptional cascade of two meiotic phases of Saccharomyces cerevisiae with members of the phylum Ascomycota, revealing that the transcriptional cascade governing chromosomal recombination during meiosis commitment has experienced greater purifying selection pressure (P value = 0.0013, 0.0382, 0.0448, 0.0369, 0.02967, 0.04937, 0.03046, 0.03357 and < 0.00001 for Ashbya gossypii, Yarrowia lipolytica, Debaryomyces hansenii, Aspergillus fumigatus, Neurospora crassa, Kluyveromyces lactis, Schizosaccharomyces pombe, Schizosaccharomyces cryophilus, and Schizosaccharomyces octosporus, respectively). This study demarcates crucial players driving meiosis initiation and commitment and demonstrates their differential rate of evolution in budding yeast.

更新日期：2023-10-31

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