用于照明应用的增压荧光蛋白-脱铁铁蛋白共晶体,ACS Nano

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用于照明应用的增压荧光蛋白-脱铁铁蛋白共晶体
ACS Nano ( IF 15.8 ) Pub Date : 2023-10-30 , DOI: 10.1021/acsnano.3c05284
Marta Patrian ₁ , Ahmed Shaukat ₂ , Mattia Nieddu ₁ , Jesús Agustín Banda-Vázquez ₁ , Jaakko V I Timonen ₃ , Juan Pablo Fuenzalida Werner ₁ , Eduardo Anaya-Plaza ₂ , Mauri A Kostiainen ₂ , Rubén D Costa ₁

Affiliation

荧光蛋白（FP）在光电子学中的应用受到了需要有效的方案来稳定它们在设备制备和操作条件下的阻碍。高温、辐射和有机溶剂暴露等因素会导致 FP 变性，导致器件性能降低。在这里，我们重点解决与 FP 运动和快速传热相关的光生热问题。这导致设备温度约为 65 °C，导致 FP 变性并随后导致设备功能丧失。我们提出了一种 FP 稳定策略，涉及将静电自组装 FP-脱铁铁蛋白共晶集成到基于硅树脂的彩色下转换滤光片中。该方法具有三个关键成就：( i ) 设计正增压 FP (+22) 的工程策略，与其天然形式相比，不会影响光致发光和热稳定性，( ii ) 精心开发的结晶方案，可产生保留了FP 的基本光致发光特性，以及 ( iii ) 器件的工作温度大幅降低至 40 °C，与参考器件相比，Bio-HLED 的稳定性提高了 40 倍。

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Supercharged Fluorescent Protein-Apoferritin Cocrystals for Lighting Applications

The application of fluorescent proteins (FPs) in optoelectronics is hindered by the need for effective protocols to stabilize them under device preparation and operational conditions. Factors such as high temperatures, irradiation, and organic solvent exposure contribute to the denaturation of FPs, resulting in a low device performance. Herein, we focus on addressing the photoinduced heat generation associated with FP motion and rapid heat transfer. This leads to device temperatures of approximately 65 °C, causing FP-denaturation and a subsequent loss of device functionality. We present a FP stabilization strategy involving the integration of electrostatically self-assembled FP-apoferritin cocrystals within a silicone-based color down-converting filter. Three key achievements characterize this approach: (i) an engineering strategy to design positively supercharged FPs (+22) without compromising photoluminescence and thermal stability compared to their native form, (ii) a carefully developed crystallization protocol resulting in highly emissive cocrystals that retain the essential photoluminescence features of the FPs, and (iii) a strong reduction of the device’s working temperature to 40 °C, leading to a 40-fold increase in Bio-HLEDs stability compared to reference devices.

更新日期：2023-10-30

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