<br>多组学分析揭示了有色体质体球在柑橘类水果类胡萝卜素积累中的重要性,The Plant Journal

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多组学分析揭示了有色体质体球在柑橘类水果类胡萝卜素积累中的重要性
The Plant Journal ( IF 6.2 ) Pub Date : 2023-10-30 , DOI: 10.1111/tpj.16519
Yun Liu _{1,

2} , Junli Ye ₁ , Man Zhu ₁ , Ross G Atkinson ₃ , Yingzi Zhang ₁ , Xiongjie Zheng ₁ , Jiao Lu ₁ , Zhen Cao ₁ , Jun Peng ₁ , Chunmei Shi ₁ , Zongzhou Xie ₁ , Robert M Larkin ₁ , Niels J Nieuwenhuizen ₃ , Charles Ampomah-Dwamena ₃ , Chuanwu Chen ₄ , Rui Wang ₅ , Xiaozhou Luo ₂ , Yunjiang Cheng ₁ , Xiuxin Deng ₁ , Yunliu Zeng ₁

Affiliation

有色体充当类胡萝卜素的代谢库，其中质体球充当多功能脂蛋白颗粒。叶绿体中的 PG 已被表征。然而，对来自非光合质体的 PG 的特征知之甚少。我们发现柑橘球状和晶体状有色体中有色体质体球（CPG）的发育与类胡萝卜素储存的改变有关。使用 Nycodenz 密度梯度超速离心，建立了一种从甜橙 ( Citrus sinensis ) 果肉中分离高纯度 CPG 的有效方案。使用比较蛋白质组学分析，44 种蛋白质被定义为可能构成 CPG 的核心蛋白质组。不同色质体微区室的脂质组分析表明，CPG 内的非极性微环境被 35 种三酰甘油酯、两种谷甾醇酯和一种豆甾醇酯修饰。在柑橘愈伤组织中操纵 CPG 定位基因CsELT1 （酯酶/脂酶/硫酯酶）导致脂质和类胡萝卜素增加，这进一步证明 CPG 的非极性微环境有助于类胡萝卜素在有色体中的积累和储存。这种 CPG 的多特征分析为有色体在类胡萝卜素代谢中的作用提供了新的线索，为操纵柑橘类水果和其他作物中的类胡萝卜素含量铺平了道路。

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Multi-omics analyses reveal the importance of chromoplast plastoglobules in carotenoid accumulation in citrus fruit

Chromoplasts act as a metabolic sink for carotenoids, in which plastoglobules serve as versatile lipoprotein particles. PGs in chloroplasts have been characterized. However, the features of PGs from non-photosynthetic plastids are poorly understood. We found that the development of chromoplast plastoglobules (CPGs) in globular and crystalloid chromoplasts of citrus is associated with alterations in carotenoid storage. Using Nycodenz density gradient ultracentrifugation, an efficient protocol for isolating highly purified CPGs from sweet orange (Citrus sinensis) pulp was established. Forty-four proteins were defined as likely comprise the core proteome of CPGs using comparative proteomics analysis. Lipidome analysis of different chromoplast microcompartments revealed that the nonpolar microenvironment within CPGs was modified by 35 triacylglycerides, two sitosterol esters, and one stigmasterol ester. Manipulation of the CPG-localized gene CsELT1 (esterase/lipase/thioesterase) in citrus calli resulted in increased lipids and carotenoids, which is further evidence that the nonpolar microenvironment of CPGs contributes to carotenoid accumulation and storage in the chromoplasts. This multi-feature analysis of CPGs sheds new light on the role of chromoplasts in carotenoid metabolism, paving the way for manipulating carotenoid content in citrus fruit and other crops.

更新日期：2023-10-30

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