热处理过程中选定的芳香化合物与肌原纤维蛋白、肌浆蛋白和胶原蛋白的结合：构象变化和蛋白质降解的作用,Journal of Agricultural and Food Chemistry

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热处理过程中选定的芳香化合物与肌原纤维蛋白、肌浆蛋白和胶原蛋白的结合：构象变化和蛋白质降解的作用
Journal of Agricultural and Food Chemistry ( IF 5.7 ) Pub Date : 2023-10-26 , DOI: 10.1021/acs.jafc.3c02618
Tianze Wang ₁ , Dong Han ₁ , Laiyu Zhao ₁ , Feng Huang ₁ , Ping Yang ₁ , Chunhui Zhang _{1,

2}

Affiliation

研究加热过程中肌原纤维蛋白（MP）、肌浆蛋白（SP）和胶原蛋白（CO）的构象变化和热降解对芳香化合物结合能力的影响。使用 SDS-PAGE、HPLC 和 LC-MS/MS，观察到蛋白质热降解形成的肽和游离氨基酸的总浓度持续上升。蛋白质的表面疏水性、总巯基含量、粒径和二级结构含量随时间发生显着变化。此外，通过气相色谱-质谱法测定蛋白质的香气结合能力。结果显示，由于蛋白质解折叠和降解产物的积累，在加热 5 或 10 分钟期间结合能力增加。然而，长时间加热会导致蛋白质聚集，结合能力下降。值得注意的是，所有蛋白质都对 ( E )-2-辛烯醛、( E , E )-2,4-癸烯醛、2-甲基-3-呋喃硫醇和二甲基三硫醚表现出强亲和力。MP和SP的结合能力相似，但与CO的结合能力显着不同，与CO相比，CO对己醛、( E )-2-辛烯醛、( E , E )-2,4-癸烯醛和二甲基三硫醚的结合能力较低MP 和 SP。

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Binding of Selected Aroma Compounds to Myofibrillar Protein, Sarcoplasmic Protein, and Collagen during Thermal Treatment: Role of Conformational Changes and Degradation of Proteins

To investigate the effects of conformational changes and thermal degradation of myofibrillar protein (MP), sarcoplasmic protein (SP), and collagen (CO) on the binding ability for aroma compounds during heating. Using SDS-PAGE, HPLC, and LC–MS/MS, a consistent rise in the total concentration of peptides and free amino acids formed by the thermal degradation of proteins was observed. The surface hydrophobicity, total sulfhydryl content, particle size, and secondary structure content of proteins changed significantly over time. Furthermore, the aroma binding ability of proteins was determined by gas chromatography–mass spectrometry. The results revealed an increase in binding ability during 5 or 10 min of heating due to protein unfolding and the accumulation of degradation products. However, the binding ability decreased due to protein aggregation with prolonged heating. Notably, all proteins exhibited strong affinity toward (E)-2-octenal, (E,E)-2,4-decadienal, 2-methyl-3-furanthiol, and dimethyl trisulfide. The binding ability of MP and SP was similar but differed significantly from that of CO, which had lower binding ability for hexanal, (E)-2-octenal, (E,E)-2,4-decadienal, and dimethyl trisulfide compared to MP and SP.

更新日期：2023-10-26

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