A promising technology for partial nitritation/anammox (PN/A) processes to treat ammonium wastewater is integrated fixed-film activated sludge (IFAS). For practical applications, achieving efficient enrichment of anammox bacteria (AnAOB) remains a challenge. In this study, membranes were temporarily used to separate solid and liquid components to induce changes in the mixed liquor suspended solids of the flocs. With membrane separation, AnAOB proliferated rapidly with a seven-fold increase in the maximum specific growth rate (μ) (from 0.009 to 0.072 d⁻¹) and a three-fold increase in the nitrogen removal rate (from 0.91 to 3.20 kg N/(m³·d)). Moreover, microbial community analysis showed significant changes in bacterial species richness and diversity with and without membrane separation. Overall, the regulation of flocs significantly influenced the microbial community structure of both flocs and biofilms leading to improved nitrogen removal efficiency in the IFAS-PN/A system.

集成固定膜活性污泥 (IFAS) 是处理含铵废水的部分亚硝化/厌氧氨氧化 (PN/A) 工艺的一项有前景的技术。对于实际应用来说，实现厌氧氨氧化细菌（AnAOB）的高效富集仍然是一个挑战。在这项研究中，暂时使用膜来分离固体和液体成分，以诱导混合液中絮凝体悬浮固体的变化。通过膜分离，AnAOB 增殖迅速，最大比生长率 (μ) 增加了 7 倍（从 0.009 到 0.072 d ^-1），脱氮率增加了 3 倍（从 0.91 到 3.20 kg N/ (m ³ ·d))。此外，微生物群落分析显示，无论是否进行膜分离，细菌物种丰富度和多样性都发生了显着变化。总体而言，絮体的调节显着影响了絮体和生物膜的微生物群落结构，从而提高了 IFAS-PN/A 系统的脱氮效率。