Customization of an Ultrafast Thiol–Norbornene Photo-Cross-Linkable Hyaluronic Acid–Gelatin Bioink for Extrusion-Based 3D Bioprinting,Biomacromolecules

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Customization of an Ultrafast Thiol–Norbornene Photo-Cross-Linkable Hyaluronic Acid–Gelatin Bioink for Extrusion-Based 3D Bioprinting
Biomacromolecules ( IF 5.5 ) Pub Date : 2023-10-26 , DOI: 10.1021/acs.biomac.3c00887
Xiong Xiao ₁ , Yuchu Yang ₁ , Yushang Lai ₁ , Ziwei Huang ₁ , Chenxi Li ₁ , Shaojie Yang ₁ , Chuan Niu ₁ , Liping Yang ₁ , Li Feng ₁

Affiliation

Light-based three-dimensional (3D) bioprinting has been widely studied in tissue engineering. Despite the fact that free-radical chain polymerization-based bioinks like hyaluronic acid methacrylate (HAMA) and gelatin methacryloyl (GelMA) have been extensively explored in 3D bioprinting, the thiol–ene hydrogel system has attracted increasing attention for its ability in building hydrogel scaffolds in an oxygen-tolerant and cell-friendly way. Herein, we report a superfast curing thiol–ene bioink composed of norbornene-modified hyaluronic acid (NorHA) and thiolated gelatin (GelSH) for 3D bioprinting. A new facile approach was first introduced in the synthesis of NorHA, which circumvented the cumbersome steps involved in previous works. Additionally, after mixing NorHA with macro-cross-linker GelSH, the customized NorHA/GelSH bioinks exhibited fascinating superiorities over the gold standard GelMA bioinks, such as an ultrafast curing rate (1–5 s), much lowered photoinitiator concentration (0.03% w/v), and flexible physical performances. Moreover, the NorHA/GelSH hydrogel greatly avoided excess ROS generation, which is important for the survival of the encapsulated cells. Last, compared with the GelMA scaffold, the 3D-printed NorHA/GelSH scaffold not only exhibited excellent cell viability but also guaranteed cell proliferation, revealing its superior bioactivity. In conclusion, the NorHA/GelSH system is a promising candidate for 3D bioprinting and tissue engineering applications.

中文翻译：

用于基于挤出的 3D 生物打印的超快硫醇-降冰片烯光可交联透明质酸-明胶生物墨水的定制

基于光的三维（3D）生物打印在组织工程中得到了广泛的研究。尽管基于自由基链聚合的生物墨水，如透明质酸甲基丙烯酸酯 (HAMA) 和明胶甲基丙烯酰 (GelMA) 已在 3D 生物打印中得到广泛探索，但硫醇-烯水凝胶系统因其构建水凝胶支架的能力而受到越来越多的关注以耐氧且对细胞友好的方式。在此，我们报道了一种由降冰片烯改性透明质酸（NorHA）和硫醇明胶（GelSH）组成的超快固化硫醇-烯生物墨水，用于3D生物打印。NorHA 的合成中首次引入了一种新的简便方法，避免了之前工作中的繁琐步骤。此外，将 NorHA 与大交联剂 GelSH 混合后，定制的 NorHA/GelSH 生物墨水比黄金标准 GelMA 生物墨水表现出令人着迷的优势，例如超快固化速率（1-5 秒）、大大降低的光引发剂浓度（0.03% w /v)，以及灵活的身体表演。此外，NorHA/GelSH水凝胶极大地避免了过量ROS的产生，这对于封装细胞的存活很重要。最后，与GelMA支架相比，3D打印的NorHA/GelSH支架不仅表现出优异的细胞活力，而且保证了细胞增殖，显示出其优越的生物活性。总之，NorHA/GelSH 系统是 3D 生物打印和组织工程应用的有希望的候选者。

更新日期：2023-10-26

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