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Effect of Anisotropic Structural Depth on Orientation and Differentiation Behavior of Skeletal Muscle Cells
ACS Omega ( IF 3.7 ) Pub Date : 2023-10-26 , DOI: 10.1021/acsomega.3c04981
Jianfeng Chen 1 , Xuefei Chen 1 , Yihao Ma 1 , Yiran Liu 1 , Jin Li 1 , Kai Peng 1 , Yichuan Dai 1 , Xiaoxiao Chen 1
ACS Omega ( IF 3.7 ) Pub Date : 2023-10-26 , DOI: 10.1021/acsomega.3c04981
Jianfeng Chen 1 , Xuefei Chen 1 , Yihao Ma 1 , Yiran Liu 1 , Jin Li 1 , Kai Peng 1 , Yichuan Dai 1 , Xiaoxiao Chen 1
Affiliation
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Extensive research has been conducted to examine how substrate topological factors are involved in modulating the cell behavior. Among numerous topological factors, the vital influence of the touchable depth of substrates on cell behaviors has already been extensively characterized, but the response of cells to the topological structure at untouchable depth is still elusive. Herein, the influences of substrate depth on myoblast behaviors are systematically investigated using substrates with depths ranging from touchable depth (microgrooved) to untouchable depth (microbridges). The results show that an increase in microgroove depth is accompanied by an inhibited cell spreading, an enhanced elongation, and a more obvious orientation along microgrooves. Interestingly, myoblasts located on microbridges show a more pronounced elongation with increasing culture time but a position-dependent orientation. Myoblasts on the center and parallel boundary of microbridges orient along the bridges, while myoblasts on the vertical boundary align perpendicular to the microbridges. Moreover, the differentiation results of the myoblasts indicate that the differentiated myotubes can maintain this position-dependent orientation. The simulation of the stress field in cell monolayers suggests that the position-dependent orientation is caused by the comprehensive response of myoblasts to the substrate discontinuity and substrate depth. These findings provide valuable insights into the mechanism of cell depth sensing and could inform the design of tissue engineering scaffolds for skeletal muscle and biohybrid actuation.
中文翻译：
各向异性结构深度对骨骼肌细胞定向和分化行为的影响
已经进行了广泛的研究来检查底物拓扑因素如何参与调节细胞行为。在众多拓扑因素中，基底可触及深度对细胞行为的重要影响已经被广泛表征，但细胞对不可触及深度拓扑结构的响应仍然难以捉摸。在此，使用深度范围从可接触深度（微槽）到不可接触深度（微桥）的基底，系统地研究了基底深度对成肌细胞行为的影响。结果表明，微槽深度的增加伴随着细胞铺展的抑制、伸长率的增强以及沿微槽的更明显的定向。有趣的是，随着培养时间的增加，位于微桥上的成肌细胞表现出更明显的伸长，但方向依赖于位置。微桥中心和平行边界上的成肌细胞沿着桥定向，而垂直边界上的成肌细胞垂直于微桥排列。此外，成肌细胞的分化结果表明分化的肌管可以维持这种位置依赖的方向。对单层细胞应力场的模拟表明，位置依赖性方向是由成肌细胞对基质不连续性和基质深度的综合响应引起的。这些发现为细胞深度传感机制提供了宝贵的见解，并可为骨骼肌和生物混合驱动的组织工程支架的设计提供信息。
更新日期：2023-10-26
中文翻译：
各向异性结构深度对骨骼肌细胞定向和分化行为的影响
已经进行了广泛的研究来检查底物拓扑因素如何参与调节细胞行为。在众多拓扑因素中，基底可触及深度对细胞行为的重要影响已经被广泛表征，但细胞对不可触及深度拓扑结构的响应仍然难以捉摸。在此，使用深度范围从可接触深度（微槽）到不可接触深度（微桥）的基底，系统地研究了基底深度对成肌细胞行为的影响。结果表明，微槽深度的增加伴随着细胞铺展的抑制、伸长率的增强以及沿微槽的更明显的定向。有趣的是，随着培养时间的增加，位于微桥上的成肌细胞表现出更明显的伸长，但方向依赖于位置。微桥中心和平行边界上的成肌细胞沿着桥定向，而垂直边界上的成肌细胞垂直于微桥排列。此外，成肌细胞的分化结果表明分化的肌管可以维持这种位置依赖的方向。对单层细胞应力场的模拟表明，位置依赖性方向是由成肌细胞对基质不连续性和基质深度的综合响应引起的。这些发现为细胞深度传感机制提供了宝贵的见解，并可为骨骼肌和生物混合驱动的组织工程支架的设计提供信息。
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