The selective functionalization of CO is of profound significance concerning the global energy and environmental crisis. Herein, we cleverly designed and constructed a photoenzymatic system with a hierarchical hollow structure photo-enzyme integrated catalyst (nickel manganate -poly(o-phenylenediamine), NiMnO@PoPDA) by coating NiMnO with artificial thylakoid membrane (PoPDA) to mimic the characteristics of natural thylakoid membranes and combined formate dehydrogenase (FDH) to upgrade CO to formic acid. The PoPDA membrane enhances the absorption of light energy and the efficiency of electron transfer, and improves the stability of the enzyme to avoid its deactivation. Most importantly, the favorable electronic coupling and band structure between NiMnO and PoPDA separate holes and electrons to afford a MV regeneration rate of 213.59 ± 4.91 mmol h g and a formic acid yield of 206 µmol. This work creates an efficient strategy using PoPDA as protector for enzyme stabilization to fabricate photobiocatalysts that realize highly efficient photoenzymatic catalysis.

中文翻译：

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人工类囊体膜辅助 NiMn2O4@PoPDA 分级空心纳米球用于光酶集成催化

CO的选择性功能化对于全球能源和环境危机具有深远的意义。在此，我们通过在NiMnO上涂覆人工类囊体膜（PoPDA）来模拟光酶的特性，巧妙地设计并构建了具有分层中空结构光酶集成催化剂（锰酸镍-聚邻苯二胺，NiMnO@PoPDA）的光酶系统。天然类囊体膜和组合甲酸脱氢酶（FDH）将CO升级为甲酸。 PoPDA膜增强了光能的吸收和电子转移的效率，并提高了酶的稳定性以避免其失活。最重要的是，NiMnO 和 PoPDA 之间良好的电子耦合和能带结构将空穴和电子分开，从而提供 213.59 ± 4.91 mmol hg 的 MV 再生率和 206 µmol 的甲酸产率。这项工作创建了一种有效的策略，使用 PoPDA 作为酶稳定的保护剂来制造实现高效光酶催化的光生物催化剂。