<br>膳食橘子通过增强肠道屏障功能、调节肠道微生物群和代谢稳态来改善小鼠抗生素相关性腹泻,Food & Function

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膳食橘子通过增强肠道屏障功能、调节肠道微生物群和代谢稳态来改善小鼠抗生素相关性腹泻
Food & Function ( IF 5.1 ) Pub Date : 2023-10-24 , DOI: 10.1039/d3fo02998k
Bin Chen _{1,

2,

3} , Xun Yang _{1,

2} , Minmin Zhan _{1,

2} , Yilu Chen ₃ , Jingyi Xu _{1,

2} , Jie Xiao _{1,

2} , Hang Xiao ₃ , Mingyue Song _{1,

2}

Affiliation

抗生素相关性腹泻是由抗生素治疗介导的，通常是由肠道屏障、肠道微生物群和代谢平衡破坏引起的。为了确定可以减轻抗生素副作用的饮食策略，本研究研究了橘皮素对 C57BL/6 小鼠抗生素相关性腹泻的影响。结果显示，饮食中的橘子素可显着改善抗生素相关性腹泻的症状，腹泻状态评分降低、粪便含水量降低、盲肠/体重比降低以及结肠组织损伤减轻就证明了这一点。膳食橘皮素还通过上调claudin-1和ZO-1的mRNA和蛋白表达对肠道屏障功能具有保护作用。此外，使用 16S rRNA 基因测序对肠道微生物群进行分析表明，膳食橘皮素通过增加肠道微生物群多样性和有益细菌（例如乳杆菌科和瘤胃球菌科）的丰度并降低丰度来调节抗生素相关性腹泻小鼠的肠道微生物群。有害细菌，例如肠球菌和球菌。此外，饮食中的橘子素可以恢复短链脂肪酸的水平，并通过丰富癌症中的嘌呤代谢、胆汁酸代谢、ABC转运蛋白和胆碱代谢来调节代谢途径。总的来说，这些发现为合理使用橘皮素作为抗生素相关性腹泻的预防和治疗药物提供了坚实的科学依据。

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Dietary tangeretin improved antibiotic-associated diarrhea in mice by enhancing the intestinal barrier function, regulating the gut microbiota, and metabolic homeostasis

Antibiotic-associated diarrhea is mediated by antibiotic treatment and is usually caused by the disruption of the intestinal barrier, gut microbiota, and metabolic balance. To identify a dietary strategy that can mitigate the side effects of antibiotics, this study investigated the effect of tangeretin on antibiotic-associated diarrhea in C57BL/6 mice. The results revealed that dietary tangeretin significantly ameliorated symptoms of antibiotic-associated diarrhea, as evidenced by the decreased diarrhea status scores, the reduced fecal water content, the decreased caecum/body weight ratio, and the alleviated colonic tissue damage. Dietary tangeretin also exhibited a protective effect on the intestinal barrier function by upregulating the mRNA and protein expression of claudin-1 and ZO-1. Furthermore, analysis of the gut microbiota using 16S rRNA gene sequencing indicated that dietary tangeretin modulated the gut microbiota of mice with antibiotic-associated diarrhea via increasing the gut microbiota diversity and the abundance of beneficial bacteria, e.g., Lactobacillaceae and Ruminococcaceae, and decreasing the abundance of harmful bacteria, e.g., Enterococcus and Terrisporobacter. Additionally, dietary tangeretin restored the levels of short-chain fatty acids and modulated metabolic pathways by enriching purine metabolism, bile acid metabolism, ABC transporters, and choline metabolism in cancer. Collectively, these findings provide a solid scientific basis for the rational use of tangeretin as a preventive and therapeutic agent for antibiotic-associated diarrhea.

更新日期：2023-10-24

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