Autonomic Neuroscience ( IF 3.2 ) Pub Date : 2023-10-21 , DOI: 10.1016/j.autneu.2023.103127 Jin Chen 1 , Kohlton T Bendowski 1 , Ariege Bizanti 1 , Yuanyuan Zhang 1 , Jichao Ma 1 , Donald B Hoover 2 , David Gozal 3 , Kalyanam Shivkumar 4 , Zixi Jack Cheng 1
Calcitonin gene-related peptide (CGRP) is widely used as a marker for nociceptive afferent axons. However, the distribution of CGRP-IR axons has not been fully determined in the whole rat heart. Immunohistochemically labeled flat-mounts of the right and left atria and ventricles, and the interventricular septum (IVS) in rats for CGRP were assessed with a Zeiss imager to generate complete montages of the entire atria, ventricles, and septum, and a confocal microscope was used to acquire detailed images of selected regions. We found that 1) CGRP-IR axons extensively innervated all regions of the atrial walls including the sinoatrial node region, auricles, atrioventricular node region, superior/inferior vena cava, left pre-caval vein, and pulmonary veins. 2) CGRP-IR axons formed varicose terminals around individual neurons in some cardiac ganglia but passed through other ganglia without making appositions with cardiac neurons. 3) Varicose CGRP-IR axons innervated the walls of blood vessels. 4) CGRP-IR axons extensively innervated the right/left ventricular walls and IVS. Our data shows the rather ubiquitous distribution of CGRP-IR axons in the whole rat heart at single-cell/axon/varicosity resolution for the first time. This study lays the foundation for future studies to quantify the differences in CGRP-IR axon innervation between sexes, disease models, and species.
中文翻译:
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降钙素基因相关肽 (CGRP) 神经支配在全大鼠心房和心室平位的分布和形态
降钙素基因相关肽 (CGRP) 被广泛用作伤害性传入轴突的标志物。然而,CGRP-IR 轴突在整个大鼠心脏中的分布尚未完全确定。 使用 Zeiss 成像仪评估大鼠免疫组织化学标记的右心房和左心房和心室平面支架以及 CGRP 的室间隔 (IVS),以生成整个心房、心室和隔膜的完整蒙太奇,并使用共聚焦显微镜获取选定区域的详细图像。我们发现 1) CGRP-IR 轴突广泛支配心房壁的所有区域,包括窦房结区、耳廓、房室结区、上/下腔静脉、左腔前静脉和肺静脉。2) CGRP-IR 轴突在一些心脏神经节的单个神经元周围形成静脉曲张末梢,但穿过其他神经节而不与心脏神经元并列。3) 静脉曲张 CGRP-IR 轴突支配血管壁。4) CGRP-IR 轴突广泛支配右/左心室壁和 IVS。我们的数据首次以单细胞/轴突/静脉曲张分辨率显示 CGRP-IR 轴突在整个大鼠心脏中相当普遍的分布。这项研究为未来量化性别、疾病模型和物种之间 CGRP-IR 轴突支配差异的研究奠定了基础。