Hydrogen sulfide (H₂S) has a crucial impact on diverse biological processes and has been shown to be related to various diseases. Many probes have been developed to detect intracellular H₂S by fluorescent imaging. However, the development of rapid, highly selective and sensitive H₂S probes remains a challenge. Herein, two fluorogenic probes, CNS and FCS, are designed and synthesized for the ultrafast detection of H₂S with fluorescein and coumarin fluorophores. The results show that both probes can be applied to monitor and image endogenous H₂S in cervical cancer HeLa cells and live zebrafish, and FCS shows a higher sensitivity, selectivity and fluorescence intensity. We then further applied FCS in a Parkinson's disease Drosophila model, and the results show that FCS can precisely indicate the level of H₂S in the Parkinson's disease model. Thus, FCS will likely to be applied for the early diagnosis of Parkinson’s disease.

硫化氢 (H ₂ S) 对多种生物过程具有至关重要的影响，并已被证明与多种疾病有关。已开发出许多探针通过荧光成像检测细胞内H ₂ S。然而，开发快速、高选择性和灵敏的H ₂ S探针仍然是一个挑战。在此，设计并合成了两种荧光探针CNS和FCS，用于使用荧光素和香豆素荧光团超快速检测 H ₂ S。结果表明，两种探针均可应用于宫颈癌HeLa细胞和活体斑马鱼内源性H _{2 S的监测和成像，且}FCS表现出较高的灵敏度、选择性和荧光强度。我们进一步将FCS应用到帕金森病果蝇模型中，结果表明FCS可以准确指示帕金森病模型中H ₂ S的水平。因此，FCS将有可能应用于帕金森病的早期诊断。