DNA 发夹和金纳米粒子的稳定性：茎长度和立足点组成的影响,Langmuir

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DNA 发夹和金纳米粒子的稳定性：茎长度和立足点组成的影响
Langmuir ( IF 3.7 ) Pub Date : 2023-10-18 , DOI: 10.1021/acs.langmuir.3c01748
Jessica S Y Ooi ₁ , Cher Ryn Lim ₁ , Chai Xian Hua ₁ , Jeck Fei Ng ₂ , Siu Yee New ₁

Affiliation

本研究研究了 DNA 发夹对无标记比色生物传感器中金纳米粒子 (AuNP) 抗盐诱导聚集 (SIA) 的稳定性的影响。AuNPs 与不同茎长度和立足点序列的 DNA 发夹一起孵育，然后添加 NaCl，然后进行紫外可见 (UV-vis) 测量。结果表明，具有较长茎的发夹通常可以提供更好的 AuNP 稳定性（18-bp > 14-bp > 10-bp）。对于立足点超过 8A 的 14-和 18-bp 发夹没有观察到任何改善，这可能归因于金表面上发夹的饱和吸附。对于具有 8 聚体均聚物立足点的 14 bp 发夹，我们观察到 A > C > G > T 的稳定趋势，与报道的 ssDNA 趋势类似。对于含有 ≥50% 腺嘌呤作为末端碱基的变体，引入胞嘧啶或鸟嘌呤作为前面的碱基也可以产生很强的稳定性。随着腺嘌呤比例的减少，鸟嘌呤或胸腺嘧啶的变体对 SIA 的保护作用减弱，特别是对于可以形成 G-四链体的富含鸟嘌呤的发夹 (≥6G)。这些发现可以作为研究人员为无标记 AuNP 生物传感器设计合适的 DNA 发夹的指南。

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DNA Hairpins and Stabilization of Gold Nanoparticles: Effect of Stem Length and Toehold Composition

This study investigates the effect of DNA hairpins on the stabilization of gold nanoparticles (AuNPs) against salt-induced aggregation (SIA) in label-free colorimetric biosensors. AuNPs were incubated with DNA hairpins of varying stem lengths and toehold sequences, followed by the addition of NaCl, before being subjected to ultraviolet–visible (UV–vis) measurement. Results showed that hairpins with longer stems generally provide better stabilization of AuNPs (18-bp >14-bp >10-bp). No improvement was observed for 14- and 18-bp hairpins with a toehold beyond 8A, which may be attributed to saturated adsorption of hairpins on the gold surface. For 14-bp hairpins with an 8-mer homopolymeric toehold, we observed a stabilization trend of A > C > G > T, similar to the reported trend of ssDNA. For variants containing ≥50% adenine as terminal bases, introducing cytosine or guanine as preceding bases could also result in strong stabilization. As the proportion of adenine decreases, variants with guanine or thymine provide less protection against SIA, especially for guanine-rich hairpins (≥6G) that could form G-quadruplexes. Such findings could serve as guidelines for researchers to design suitable DNA hairpins for label-free AuNP-based biosensors.

更新日期：2023-10-18

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