丘脑室旁核前后轴转录组和连接性的独特性和连续性,Cell Reports

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丘脑室旁核前后轴转录组和连接性的独特性和连续性
Cell Reports ( IF 7.5 ) Pub Date : 2023-10-19 , DOI: 10.1016/j.celrep.2023.113309
Yasuyuki Shima ₁ , Henrik Skibbe ₂ , Yohei Sasagawa ₃ , Noriko Fujimori ₄ , Yoshimi Iwayama ₃ , Ayako Isomura-Matoba ₅ , Minoru Yano ₆ , Takumi Ichikawa ₃ , Itoshi Nikaido ₃ , Nobutaka Hattori ₇ , Tadafumi Kato ₈

Affiliation

Neurodegenerative Disorders Collaborative Laboratory, RIKEN, Wako, Saitama 351-0198, Japan; Laboratory of Molecular Dynamics of Mental Disorders, RIKEN, Wako, Saitama 351-0198, Japan.
Brain Image Analysis Unit, RIKEN, Wako, Saitama 351-0198, Japan.
Laboratory for Bioinformatics Research, Center for Biosystems Dynamics Research, RIKEN, Wako, Saitama 351-0198, Japan; Department of Functional Genome Informatics, Division of Biological Data Science, Medical Research Institute, Tokyo Medical and Dental University (TMDU), Hongo, Bunkyo City, Tokyo 113-8519, Japan.
Laboratory of Molecular Dynamics of Mental Disorders, RIKEN, Wako, Saitama 351-0198, Japan; Support Unit for Bio-Material Analysis, Research Resource Division, Center for Brain Science, RIKEN, Wako, Saitama 351-0198, Japan.
Laboratory for Bioinformatics Research, Center for Biosystems Dynamics Research, RIKEN, Wako, Saitama 351-0198, Japan.
Department of Functional Genome Informatics, Division of Biological Data Science, Medical Research Institute, Tokyo Medical and Dental University (TMDU), Hongo, Bunkyo City, Tokyo 113-8519, Japan.
Neurodegenerative Disorders Collaborative Laboratory, RIKEN, Wako, Saitama 351-0198, Japan; Department of Neurology, Juntendo University, Hongo, Bunkyo City, Tokyo 113-8421, Japan.
Laboratory of Molecular Dynamics of Mental Disorders, RIKEN, Wako, Saitama 351-0198, Japan; Department of Psychiatry, Juntendo University, Hongo, Bunkyo City, Tokyo 113-8421, Japan; Department of Molecular Pathology of Mood Disorders, Juntendo University, Hongo, Bunkyo City, Tokyo 113-8421, Japan.

丘脑室旁核（PVT）将轴突投射到多个区域，介导广泛的行为，并在功能和轴突投射方面表现出区域异质性。尽管如此，有关 PVT 中存在的细胞类型及其差异程度的问题仍未得到充分解决。我们应用单细胞 RNA 测序来描述小鼠 PVT 神经元的转录组特征。我们发现 PVT 转录组中最显着的差异之一对应于前后轴。虽然单细胞转录组将 PVT 神经元分为五种类型，但我们的转录组和组织学分析显示了细胞类型之间的连续性。我们发现前亚群和后亚群具有几乎不重叠的投影模式，而另一个亚群则表现出中间模式。此外，这些亚群对与食欲相关的神经肽的反应不同，它们的激活对食物消耗表现出相反的影响。我们的研究揭示了支持 PVT 神经元功能的对比和连续性。

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更新日期：2023-10-19

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