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A cell-free artificial anabolic pathway for direct conversion of CO2 to ethanol
Green Chemistry ( IF 9.3 ) Pub Date : 2023-10-17 , DOI: 10.1039/d3gc03159d Wanrong Dong 1, 2 , Xiuling Ji 1 , Yuhong Huang 1 , Yaju Xue 1 , Boxia Guo 1 , Dongbo Cai 2 , Shouwen Chen 2 , Suojiang Zhang 1
Green Chemistry ( IF 9.3 ) Pub Date : 2023-10-17 , DOI: 10.1039/d3gc03159d Wanrong Dong 1, 2 , Xiuling Ji 1 , Yuhong Huang 1 , Yaju Xue 1 , Boxia Guo 1 , Dongbo Cai 2 , Shouwen Chen 2 , Suojiang Zhang 1
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Biological CO2 activation and conversion to high-value ethanol are a feasible and green strategy to close the carbon cycle. However, naturally evolved CO2 utilization pathways involve carbon loss or ATP consumption. Herein, we report a complete anabolic pathway for direct conversion of CO2 to ethanol by constructing and assembling three functional modules including CO2 activation, formaldehyde → acetyl-CoA, and ethanol synthesis in a carbon-conserved and ATP-independent system. These artificial ethanol metabolic pathway fluxes were strengthened by screening efficient key enzymes for CO2 activation, promoting formaldehyde assimilation, and employing a two-step reaction. 13C-labeled CO2 demonstrated the feasibility of the pathway converting CO2 into ethanol in vitro by detecting the carbon flow. With this anabolic pathway, we obtained an ethanol concentration of 0.37 mM at a conversion rate of 4.33 nmol CO2 min−1 mg−1 enzyme and 0.5 mM R5P supply. This modularization strategy provides a new avenue in the construction of artificial metabolic pathways for ethanol synthesis from CO2.
中文翻译:
将二氧化碳直接转化为乙醇的无细胞人工合成代谢途径
生物CO 2活化并转化为高价值乙醇是闭合碳循环的可行且绿色的策略。然而,自然进化的CO 2利用途径涉及碳损失或ATP消耗。在此,我们通过在碳保守且不依赖于ATP的系统中构建和组装三个功能模块(包括CO 2 活化、甲醛→乙酰辅酶A和乙醇合成),报告了将CO 2 直接转化为乙醇的完整合成代谢途径。通过筛选有效的CO 2活化关键酶、促进甲醛同化以及采用两步反应,增强了这些人工乙醇代谢途径通量。13 C标记的CO 2通过检测碳流证明了体外将CO 2转化为乙醇的途径的可行性。通过这种合成代谢途径,我们以 4.33 nmol CO 2 min -1 mg -1酶和 0.5 mM R5P 供应的转化率获得了 0.37 mM 的乙醇浓度。这种模块化策略为构建从CO 2合成乙醇的人工代谢途径提供了新途径。
更新日期:2023-10-17
中文翻译:
![](https://scdn.x-mol.com/jcss/images/paperTranslation.png)
将二氧化碳直接转化为乙醇的无细胞人工合成代谢途径
生物CO 2活化并转化为高价值乙醇是闭合碳循环的可行且绿色的策略。然而,自然进化的CO 2利用途径涉及碳损失或ATP消耗。在此,我们通过在碳保守且不依赖于ATP的系统中构建和组装三个功能模块(包括CO 2 活化、甲醛→乙酰辅酶A和乙醇合成),报告了将CO 2 直接转化为乙醇的完整合成代谢途径。通过筛选有效的CO 2活化关键酶、促进甲醛同化以及采用两步反应,增强了这些人工乙醇代谢途径通量。13 C标记的CO 2通过检测碳流证明了体外将CO 2转化为乙醇的途径的可行性。通过这种合成代谢途径,我们以 4.33 nmol CO 2 min -1 mg -1酶和 0.5 mM R5P 供应的转化率获得了 0.37 mM 的乙醇浓度。这种模块化策略为构建从CO 2合成乙醇的人工代谢途径提供了新途径。