Engineering of Tunable Allosteric-like Fluorogenic Protein Sensors,ACS Sensors

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Engineering of Tunable Allosteric-like Fluorogenic Protein Sensors
ACS Sensors ( IF 8.2 ) Pub Date : 2023-10-13 , DOI: 10.1021/acssensors.3c01536
Fanny Broch ₁ , Lina El Hajji ₁ , Nicolas Pietrancosta _{1,

2} , Arnaud Gautier _{1,

3}

Affiliation

Optical protein sensors that enable detection of relevant biomolecules of interest play central roles in biological research. Coupling fluorescent reporters with protein sensing units has enabled the development of a wide range of biosensors that recognize analytes with high selectivity. In these sensors, analyte recognition induces a conformational change in the protein sensing unit that can modulate the optical signal of the fluorescent reporter. Here, we explore various designs for the creation of tunable allosteric-like fluorogenic protein sensors through incorporation of sensing protein units within the chemogenetic fluorescence-activating and absorption-shifting tag (FAST) that selectively binds and stabilizes the fluorescent state of 4-hydroxybenzylidene rhodanine (HBR) analogs. Conformational coupling allowed us to design analyte-responsive optical protein sensors through allosteric-like modulation of fluorogen binding.

中文翻译：

可调谐变构样荧光蛋白传感器的工程

能够检测感兴趣的相关生物分子的光学蛋白质传感器在生物学研究中发挥着核心作用。将荧光报告基因与蛋白质传感单元耦合，能够开发出多种能够高选择性识别分析物的生物传感器。在这些传感器中，分析物识别会引起蛋白质传感单元的构象变化，从而调节荧光报告基因的光学信号。在这里，我们探索了通过将传感蛋白单元纳入化学遗传学荧光激活和吸收位移标签（FAST）中来创建可调谐变构样荧光蛋白传感器的各种设计，该标签选择性地结合并稳定 4-羟基亚苄基绕丹宁的荧光状态（哈佛商业评论）类似物。构象耦合使我们能够通过荧光素结合的变构样调节来设计分析物响应的光学蛋白质传感器。

更新日期：2023-10-13

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