具有增强原位矿化作用的仿生矿化 3D 打印支架，用于颅骨再生,Advanced Functional Materials

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具有增强原位矿化作用的仿生矿化 3D 打印支架，用于颅骨再生
Advanced Functional Materials ( IF 18.5 ) Pub Date : 2023-10-15 , DOI: 10.1002/adfm.202309042
Ling Wang _{1,

2} , Dongxuan Li _{1,

2} , Yawen Huang _{1,

2} , Ruiqi Mao ₃ , Boqing Zhang _{1,

2} , Fengxiong Luo _{1,

2} , Peiyang Gu _{1,

2} , Ping Song _{1,

2} , Xiang Ge ₄ , Jian Lu _{1,

2} , Xusheng Yang ₅ , Yujiang Fan _{1,

2} , Xingdong Zhang _{1,

2} , Kefeng Wang _{1,

2}

Affiliation

原位矿化是模拟生物矿物理化性质的一种有前景的策略，广泛应用于骨修复领域。鉴于颅骨再生对物质交换的高要求，原位矿化的有机-无机杂化材料表现出优势。然而，卓越的矿物质含量、机械性能和成骨性能的整合仍然是一个重大挑战。在此，通过结合酶促矿化和阴离子促进矿化来增强原位矿化，以提高矿化效率、矿物含量和机械性能。基于计算计算和体外矿化实验结果，从成核位点和原位矿化饱和度角度研究了矿化增强机理。阴离子聚天冬氨酸（pAsp）可以提高原位矿化的饱和度；酶法矿化效率高，矿物质结晶度低。体外细胞增殖/分化实验和体内颅骨再生实验证实，矿物质特性的变化有效增强了3D打印支架的生物学特性。该策略为制备用于颅骨修复的仿生矿化支架提供了新思路，可极大促进骨再生效率。

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Bionic Mineralized 3D-Printed Scaffolds with Enhanced In Situ Mineralization for Cranial Bone Regeneration

In situ mineralization is a promising strategy to mimic the physicochemical properties of biominerals and is widely applied in the field of bone repair. Given the high requirement for substance exchange in cranial bone regeneration, in situ mineralized organic–inorganic hybrid materials exhibit advantages. However, the integration of remarkable mineral content, mechanical properties, and osteogenic properties also remains a major challenge. Herein, enhanced in situ mineralization through combining the enzymatic and anion-boosted mineralization is applied to promote the mineralization efficiency, mineral content, and mechanical properties. Based on the results of computational calculations and in vitro mineralization experiments, the mechanism of mineralization enhancement is investigated from the perspectives of nucleation sites and the saturation of in situ mineralization. Anionic polyaspartic acid (pAsp) can increase the saturation of in situ mineralization; enzymatic mineralization shows high efficiency, with minerals of low crystallinity. The changes in the properties of the minerals effectively enhance the biological properties of 3D-printed scaffolds, as confirmed by cell proliferation/differentiation experiments in vitro and in cranial bone regeneration in vivo. This strategy provides a new thinking for the preparation of bionic mineralized scaffolds for cranial bone repair, and can greatly promote the efficiency of bone regeneration.

更新日期：2023-10-15

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