<br>RNase III 参与球形红杆菌群体感应、色素沉着和氧化应激抵抗的控制,Molecular Microbiology

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RNase III 参与球形红杆菌群体感应、色素沉着和氧化应激抵抗的控制
Molecular Microbiology ( IF 2.6 ) Pub Date : 2023-10-12 , DOI: 10.1111/mmi.15181
Janek Börner ₁ , Tobias Friedrich _{2,

3} , Gabriele Klug ₁

Affiliation

RNase III 是一种 dsRNA 特异性内切核糖核酸酶，在细菌和真核生物中高度保守。在本研究中，我们分析了 RNase III 失活对兼性光养 α-变形菌球形红杆菌转录组和表型的影响。 RNA-seq 揭示了位于 rRNA 操纵子直接下游的表达量增加的基因数量出乎意料地高。额外转录终止子的染色体插入恢复了下游基因的野生型样表达，表明 RNase III 可能调节球形R. sphaeroides中的 rRNA 转录终止。此外，我们确定 RNase III 是球形红球菌群体感应自诱导剂合成的主要调节因子。它通过降低cerI mRNA 稳定性来负向控制自诱导剂合酶 CerI 的表达。此外，RNase III 失活导致氧化应激抵抗力改变，并损害光合活性色素-蛋白质复合物的形成。我们还观察到 CcsR 小 RNA 的增加，此前已证明这些小 RNA 可以促进对氧化应激的抵抗力。总而言之，我们的数据提供了对 RNase III 介导的调节的有趣见解，并扩展了关于这种重要酶在细菌中的功能的知识。

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RNase III participates in control of quorum sensing, pigmentation and oxidative stress resistance in Rhodobacter sphaeroides

RNase III is a dsRNA-specific endoribonuclease, highly conserved in bacteria and eukarya. In this study, we analysed the effects of inactivation of RNase III on the transcriptome and the phenotype of the facultative phototrophic α-proteobacterium Rhodobacter sphaeroides. RNA-seq revealed an unexpectedly high amount of genes with increased expression located directly downstream to the rRNA operons. Chromosomal insertion of additional transcription terminators restored wild type-like expression of the downstream genes, indicating that RNase III may modulate the rRNA transcription termination in R. sphaeroides. Furthermore, we identified RNase III as a major regulator of quorum-sensing autoinducer synthesis in R. sphaeroides. It negatively controls the expression of the autoinducer synthase CerI by reducing cerI mRNA stability. In addition, RNase III inactivation caused altered resistance against oxidative stress and impaired formation of photosynthetically active pigment-protein complexes. We also observed an increase in the CcsR small RNAs that were previously shown to promote resistance to oxidative stress. Taken together, our data present interesting insights into RNase III-mediated regulation and expand the knowledge on the function of this important enzyme in bacteria.

更新日期：2023-10-12

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