Cell-selective bioorthogonal labeling,Cell Chemical Biology

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Cell-selective bioorthogonal labeling
Cell Chemical Biology ( IF 6.6 ) Pub Date : 2023-10-13 , DOI: 10.1016/j.chembiol.2023.09.010
Wei Huang ₁ , Scott T Laughlin ₁

Affiliation

In classic bioorthogonal labeling experiments, the cell’s biosynthetic machinery incorporates bioorthogonal tags, creating tagged biomolecules that are subsequently reacted with a corresponding bioorthogonal partner. This two-step approach labels biomolecules throughout the organism indiscriminate of cell type, which can produce background in applications focused on specific cell populations. In this review, we cover advances in bioorthogonal chemistry that enable targeting of bioorthogonal labeling to a desired cell type. Such cell-selective bioorthogonal labeling is achieved in one of three ways. The first approach restricts labeling to specific cells by cell-selective expression of engineered enzymes that enable the bioorthogonal tag’s incorporation. The second approach preferentially localizes the bioorthogonal reagents to the desired cell types to restrict their uptake to the desired cells. Finally, the third approach cages the reactivity of the bioorthogonal reagents, allowing activation of the reaction in specific cells by uncaging the reagents selectively in those cell populations.

中文翻译：

细胞选择性生物正交标记

在经典的生物正交标记实验中，细胞的生物合成机制包含生物正交标签，产生标记的生物分子，随后与相应的生物正交伙伴发生反应。这种两步方法在整个生物体中不分细胞类型地标记生物分子，这可以在专注于特定细胞群的应用中产生背景。在这篇综述中，我们介绍了生物正交化学的进展，这些进展使得生物正交标记能够靶向所需的细胞类型。这种细胞选择性生物正交标记是通过三种方式之一实现的。第一种方法通过工程酶的细胞选择性表达来限制对特定细胞的标记，从而实现生物正交标签的掺入。第二种方法优先将生物正交试剂定位于所需的细胞类型，以限制其对所需细胞的摄取。最后，第三种方法限制了生物正交试剂的反应性，通过选择性地释放这些细胞群中的试剂来激活特定细胞中的反应。

更新日期：2023-10-13

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