Many studies have been conducted on the transduction efficiency of recombinant adeno-associated virus (rAAV) depending on the serotype and genome structure, such as single-stranded (ss) and self-complementary (sc). To understand the variation in therapeutic efficacy, we focused on investigating subcellular distribution of viral genome depending on rAAV genome structure. It is critical to ascertain the location of the virus within the host cell after the entry because a larger amount of the viral genome placed in the nucleus facilitates viral genome replication by utilizing the host cell's system, thereby enhancing the therapeutic outcome. In this sense, tracking the location of the virus within the host cell's organelles can inform a new strategy to improve therapeutic efficacy. Therefore, we attempted to stain only the viral genome with APEX2 and DAB chemicals specifically, and the distribution of the viral genome was examined by transmission electron microscopy (TEM). Consequently, when the two types of rAAV were transduced for 6 h, scAAV2 tended to be more located in the lysosome and nucleus compared to ssAAV2.

人们对重组腺相关病毒（rAAV）的转导效率进行了许多研究，具体取决于血清型和基因组结构，例如单链（ss）和自身互补（sc）。为了了解治疗效果的变化，我们重点研究了取决于 rAAV 基因组结构的病毒基因组的亚细胞分布。确定病毒进入宿主细胞后的位置至关重要，因为大量的病毒基因组位于细胞核中，可利用宿主细胞的系统促进病毒基因组复制，从而增强治疗效果。从这个意义上说，追踪病毒在宿主细胞细胞器内的位置可以为提高治疗效果的新策略提供信息。因此，我们尝试仅用 APEX2 和 DAB 化学物质对病毒基因组进行特异性染色，并通过透射电子显微镜 (TEM) 检查病毒基因组的分布。因此，当两种类型的rAAV转导6小时时，与ssAAV2相比，scAAV2更倾向于位于溶酶体和细胞核中。