大蒜素可减轻丙烯酰胺诱导的大鼠肾脏中涉及 SIRT1 调节的线粒体功能障碍,Journal of Agricultural and Food Chemistry

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大蒜素可减轻丙烯酰胺诱导的大鼠肾脏中涉及 SIRT1 调节的线粒体功能障碍
Journal of Agricultural and Food Chemistry ( IF 5.7 ) Pub Date : 2023-10-13 , DOI: 10.1021/acs.jafc.3c04687
Lu Li ₁ , Ziyue Wang ₁ , Yucai Li ₁ , Fuying Ma ₁ , Xinwei Wang ₁ , Haiyang Yan ₁ , Wenliang Li ₁ , Yong Pang ₁ , Yuan Yuan ₁

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丙烯酰胺（AA）通常在富含碳水化合物的热处理食品中形成，对肾脏产生有害影响。大蒜素来自压碎的蒜瓣，具有很强的生物活性。本研究基于大蒜素在改善肾功能中发挥关键作用的前期研究，利用体内大鼠模型，全面探讨了大蒜素对AA引起的肾毒性的保护机制。结果表明，大蒜素减轻了肾脏的组织学变化并改善了肾功能。AA 处理后观察到线粒体结构受损、电压依赖性阴离子通道 1 表达上调、膜电位和 5'-三磷酸腺苷水平降低。令人惊讶的是，大蒜素显着逆转了不利影响。此外，大蒜素通过调节线粒体生物合成和动力学有效恢复线粒体功能，这可能与上调 Sirtuin 1 (SIRT1) 的表达有关。同时，大蒜素显着激活SIRT1活性，随后抑制p53乙酰化，阻止细胞色素c向细胞质转位，并减少caspase表达，从而进一步抑制AA引起的线粒体凋亡。综上所述，大蒜素对AA所致肾毒性的缓解作用在于其抑制线粒体功能障碍和线粒体凋亡。

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Allicin Alleviates Mitochondrial Dysfunction in Acrylamide-Induced Rat Kidney Involving the Regulation of SIRT1

Acrylamide (AA), commonly formed in carbohydrate-rich thermally processed foods, exerts harmful effects on the kidney. Allicin, from crushed garlic cloves, exhibits strong biological activities. In the current study, the protection mechanisms of allicin against AA-caused nephrotoxicity were comprehensively examined using an in vivo rat model based on previous research that allicin plays a key role in improving renal function. The results showed that allicin attenuated histological changes of the kidney and ameliorated renal function. Damaged mitochondrial structures, upregulated voltage-dependent anion channel 1 expression, and decreased membrane potential and adenosine 5′-triphosphate levels were observed after AA treatment. Surprisingly, allicin notably reversed the adverse effects. Further, allicin effectively restored mitochondrial function via modulating mitochondrial biogenesis and dynamics, which might be associated with the upregulated expression of sirtuin 1 (SIRT1). Meanwhile, allicin dramatically activated the SIRT1 activity and subsequently inhibited p53 acetylation, prevented the translocation of cytochrome c to the cytoplasm, and reduced the caspase expression, thus further inhibiting mitochondrial apoptosis caused by AA. In summary, the relieving effect of allicin on AA-caused nephrotoxicity lies in its inhibition of mitochondrial dysfunction and mitochondrial apoptosis.

更新日期：2023-10-13

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