Itaconic acid is a promising biochemical building block that can be used in polymer synthesis. Itaconic acid is currently produced in industry by the natural producer fungus Aspergillus terreus using glucose as a main carbon source. Most research for itaconic acid production using lignocellulosic-based carbon sources was carried out by A. terreus. Engineered Corynebacterium glutamicum strain which can grow in presence of fermentation inhibitors without effect on growth, was used for production of itaconic acid using sweet sorghum juice and bagasse sugar lysate (BSL). BSL contains many inhibitors unlike sorghum juice. C. glutamicum could grow in the media containing both types of lignocellulose-based carbon sources without showing any growth inhibition, however, sorghum juice was better in itaconic acid production than BSL. Different constructed strains of C. glutamicum were used for itaconic acid production, however, C. glutamicum ATCC 13032 pCH-Tad1_optAdi1_opt strain expressing Adi1/Tad1 genes (trans-pathway) from Ustilago maydis proved to be better in itaconic acid production giving final titer of 8.4 and 4.02 g/L using sweet sorghum juice and BSL as the sole carbon sources by fed-batch fermentation. Our study is the first for production of itaconic acid using sweet sorghum juice and BSL. The present study also proved that C. glutamicum can be used for enhancing itaconic acid production using lignocellulosic-based carbon sources.

衣康酸是一种有前途的生化结构单元，可用于聚合物合成。衣康酸目前在工业上由天然生产真菌土曲霉使用葡萄糖作为主要碳源生产。大多数使用木质纤维素基碳源生产衣康酸的研究是由A. terreus进行的。工程谷氨酸棒杆菌菌株可以在发酵抑制剂存在下生长而不影响生长，用于使用甜高粱汁和甘蔗渣糖裂解物（BSL）生产衣康酸。与高粱汁不同，BSL 含有许多抑制剂。谷氨酸棒杆菌可以在含有两种类型的基于木质纤维素的碳源的培养基中生长，而没有表现出任何生长抑制，然而，高粱汁在衣康酸生产方面比BSL更好。不同构建的谷氨酸棒杆菌菌株用于衣康酸生产，然而，来自玉米黑粉菌的表达Adi1 / Tad1基因（反式途径）的谷氨酸棒杆菌ATCC 13032 pCH - Tad1 _opt Adi1 _opt菌株被证明在衣康酸生产方面表现更好使用甜高粱汁和 BSL 作为唯一碳源，通过补料分批发酵，最终效价分别为 8.4 和 4.02 g/L。我们的研究是第一个利用甜高粱汁和 BSL 生产衣康酸的研究。本研究还证明谷氨酸棒杆菌可用于利用木质纤维素基碳源提高衣康酸产量。