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Visualization of Lysosomal Dynamics during Autophagy by Fluorescent Probe
Analytical Chemistry ( IF 6.7 ) Pub Date : 2023-10-10 , DOI: 10.1021/acs.analchem.3c03329 Guofang Li 1 , Langdi Zhang 1 , Hua Zheng 1 , Ling Huang 1 , Zihong Li 1 , Wenxiu Li 1 , Weiying Lin 1
Analytical Chemistry ( IF 6.7 ) Pub Date : 2023-10-10 , DOI: 10.1021/acs.analchem.3c03329 Guofang Li 1 , Langdi Zhang 1 , Hua Zheng 1 , Ling Huang 1 , Zihong Li 1 , Wenxiu Li 1 , Weiying Lin 1
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Lysosomes are one of the important organelles within cells, and their dynamic movement processes are associated with many biological events. Therefore, real-time monitoring of lysosomal dynamics processes has far-reaching implications. A lysosome-targeted fluorescent probe N(CH2)3-BD-PZ is proposed for real-time monitoring of lysosomal kinetic motility. Using this probe, the dynamic process of lysosomes under starvation induction was successfully explored through fluorescence imaging. Importantly, we observed a new pattern of lysosomal dynamic movement, in which an irregular lysosome was slowly cleaved into two different-sized touching lysosomes and then fused to form a new round lysosome. This research provides a powerful fluorescence tool to understand the dynamic motility of intracellular lysosomes under fluorescence imaging.
中文翻译：
通过荧光探针观察自噬过程中溶酶体动力学
溶酶体是细胞内重要的细胞器之一，其动态运动过程与许多生物事件相关。因此，实时监测溶酶体动力学过程具有深远的影响。提出了一种溶酶体靶向荧光探针N(CH 2 ) 3 -BD-PZ，用于实时监测溶酶体动力学。利用该探针，通过荧光成像成功探索了饥饿诱导下溶酶体的动态过程。重要的是，我们观察到一种新的溶酶体动态运动模式，其中不规则的溶酶体慢慢分裂成两个不同大小的接触溶酶体，然后融合形成新的圆形溶酶体。这项研究为了解荧光成像下细胞内溶酶体的动态运动提供了强大的荧光工具。
更新日期：2023-10-10
中文翻译：
通过荧光探针观察自噬过程中溶酶体动力学
溶酶体是细胞内重要的细胞器之一，其动态运动过程与许多生物事件相关。因此，实时监测溶酶体动力学过程具有深远的影响。提出了一种溶酶体靶向荧光探针N(CH 2 ) 3 -BD-PZ，用于实时监测溶酶体动力学。利用该探针，通过荧光成像成功探索了饥饿诱导下溶酶体的动态过程。重要的是，我们观察到一种新的溶酶体动态运动模式，其中不规则的溶酶体慢慢分裂成两个不同大小的接触溶酶体，然后融合形成新的圆形溶酶体。这项研究为了解荧光成像下细胞内溶酶体的动态运动提供了强大的荧光工具。
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