In the present study, the mitochondrial genomes of Peltigera elisabethae and P. polydactylon were sequenced and assembled. The two mitogenomes were composed of circular DNA molecules, with sizes of 64,034 bp and 59,208 bp, respectively. Comparative analysis showed that the genome size, GC content, GC skew, and AT skew varied between the two mitochondrial genomes. In codon analysis, phenylalanine (Phe), isoleucine (Ile), and leucine (Leu) were most frequently used in six Peltigera genomes. Evolutionary analysis showed that all 14 protein-coding genes (PCGs) were subject to purifying selection in the six Peltigera species. Regarding gene rearrangement, the PCGs of Peltigera had the same gene sequence and gene content, and a few intron sequences and spacer sequences were rearranged in Peltigera. In the phylogenetic analysis, we used Bayesian and ML methods to construct a phylogenetic tree. Two phylogenetic trees with consistent topology with high support indicate that mitochondrial genes were reliable molecular markers for analyzing the phylogenetic relationships. The present study enriches the mitochondrial genome data of Peltigera and promotes further understanding of the genetics and evolution of the Peltigera genus.

在本研究中，对Peltigera elisabethae和P. polydactylon的线粒体基因组进行了测序和组装。两个有丝分裂基因组由环状DNA分子组成，大小分别为64,034 bp和59,208 bp。比较分析表明，两个线粒体基因组的基因组大小、GC 含量、GC 偏斜和 AT 偏斜有所不同。在密码子分析中，苯丙氨酸 (Phe)、异亮氨酸 (Ile) 和亮氨酸 (Leu) 在六个Peltigera基因组中最常用。进化分析表明，6 个Peltigera物种中的所有 14 个蛋白质编码基因 (PCG) 均受到纯化选择。在基因重排方面， Peltigera的PCG具有相同的基因序列和基因内容，并且Peltigera的少数内含子序列和间隔序列发生了重排。在系统发育分析中，我们使用贝叶斯和ML方法构建系统发育树。两棵具有一致拓扑和高支持度的系统发育树表明线粒体基因是分析系统发育关系的可靠分子标记。本研究丰富了Peltigera属的线粒体基因组数据，促进了对Peltigera属遗传学和进化的进一步了解。