Preeclampsia (PE) belongs to hypertensive disorder complicating pregnancy, which is a serious obstetric complication. Propofol is a new type of fast and short-acting general anesthetic, which has also been demonstrated to promote the cell growth recently. Therefore, this study was carried out to explore the effects of propofol on the cell growth, migration and invasion in the HTR-8/SVneo cells. The cell biological behaviors were analyzed using CCK-8, EdU, transwell assays. The relationship between METTL3 and ZEB2 was confirmed by RIP assay. Western blot and RT-qPCR assays were carried out to detect the protein and mRNA levels. The results showed that propofol enhanced the cell viability, proliferation, migration and invasion of the HTR-8/SVneo cells. Besides, METTL3 overexpression neutralized the propofol role. Furthermore, METTL3 overexpression elevated the m6A levels of ZEB2 and decreased the mRNA levels and stability of ZEB2. ZEB2 overexpression neutralized the role of METTL3 in the propofol treated HTR-8/SVneo cells. In conclusion, this study demonstrated the effects of propofol on promoting the cell growth, migration and invasion of HTR-8/SVneo cells. Mechanistically, propofol indirectly regulated ZEB2 expression by targeting METTL3 mediated m6A methylation modification.

先兆子痫（PE）属于妊娠期高血压疾病，是一种严重的产科并发症。异丙酚是一种新型快速、短效全身麻醉剂，最近也被证明可以促进细胞生长。因此，本研究旨在探讨异丙酚对HTR-8/SVneo细胞生长、迁移和侵袭的影响。使用CCK-8、EdU、transwell 实验分析细胞生物学行为。 METTL3 和ZEB2 之间的关系通过RIP 测定得到证实。进行蛋白质印迹和 RT-qPCR 测定来检测蛋白质和 mRNA 水平。结果表明，异丙酚增强了HTR-8/SVneo细胞的活力、增殖、迁移和侵袭。此外，METTL3的过度表达中和了异丙酚的作用。此外，METTL3过表达升高了ZEB2的m6A水平并降低了ZEB2的mRNA水平和稳定性。 ZEB2 过表达中和了异丙酚处理的 HTR-8/SVneo 细胞中 METTL3 的作用。总之，本研究证明了异丙酚对HTR-8/SVneo细胞的生长、迁移和侵袭的促进作用。从机制上讲，丙泊酚通过靶向 METTL3 介导的 m6A 甲基化修饰间接调节 ZEB2 表达。