Nanopore Signatures of Nucleoside Drugs,Nano Letters

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Nanopore Signatures of Nucleoside Drugs
Nano Letters ( IF 9.6 ) Pub Date : 2023-10-11 , DOI: 10.1021/acs.nanolett.3c02872
Wendong Jia _{1,

2} , Yusheng Ouyang _{1,

2} , Shanyu Zhang _{1,

2} , Xiaoyu Du _{1,

2} , Panke Zhang ₁ , Shuo Huang _{1,

2}

Affiliation

Nucleoside drugs, which are analogues of natural nucleosides, have been widely applied in the clinical treatment of viral infections and cancers. The development of nucleoside drugs, repurposing of existing drugs, and combined use of multiple drug types have made the rapid sensing of nucleoside drugs urgently needed. Nanopores are emerging single-molecule sensors that have high resolution to resolve even minor structural differences between chemical compounds. Here, an engineered Mycobacterium smegmatis porin A hetero-octamer was used to perform general nucleoside drug analysis. Ten nucleoside drugs were simultaneously detected and fully discriminated. An accuracy of >99.9% was consequently reported. This sensing capacity was further demonstrated in direct nanopore analysis of ribavirin buccal tablets, confirming its sensing reliability against complex samples and environments. No sample separation is needed, however, significantly minimizing the complexity of the measurement. This technique may inspire nanopore applications in pharmaceutical production and pharmacokinetics measurements.

中文翻译：

核苷类药物的纳米孔特征

核苷类药物是天然核苷的类似物，已广泛应用于病毒感染和癌症的临床治疗。核苷类药物的开发、现有药物的再利用以及多种药物类型的联合使用使得核苷类药物的快速传感迫切需要。纳米孔是新兴的单分子传感器，具有高分辨率，可以解决化合物之间即使是微小的结构差异。在这里，使用工程改造的耻垢分枝杆菌孔蛋白 A 异八聚体进行一般核苷药物分析。十种核苷类药物同时检测并充分区分。据报道，准确度>99.9%。这种传感能力在利巴韦林口腔片的直接纳米孔分析中得到了进一步证明，证实了其对复杂样品和环境的传感可靠性。然而，无需分离样品，从而大大降低了测量的复杂性。该技术可能会激发纳米孔在药物生产和药代动力学测量中的应用。

更新日期：2023-10-11

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