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Natural variation in STAYGREEN contributes to low-temperature tolerance in cucumber
Journal of Integrative Plant Biology ( IF 9.3 ) Pub Date : 2023-10-09 , DOI: 10.1111/jipb.13571 Shaoyun Dong 1 , Caixia Li 1 , Haojie Tian 1 , Weiping Wang 1 , Xueyong Yang 1 , Diane M Beckles 2 , Xiaoping Liu 1 , Jiantao Guan 1 , Xingfang Gu 1 , Jiaqiang Sun 3 , Han Miao 1 , Shengping Zhang 1
Journal of Integrative Plant Biology ( IF 9.3 ) Pub Date : 2023-10-09 , DOI: 10.1111/jipb.13571 Shaoyun Dong 1 , Caixia Li 1 , Haojie Tian 1 , Weiping Wang 1 , Xueyong Yang 1 , Diane M Beckles 2 , Xiaoping Liu 1 , Jiantao Guan 1 , Xingfang Gu 1 , Jiaqiang Sun 3 , Han Miao 1 , Shengping Zhang 1
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Low-temperature (LT) stress threatens cucumber production globally; however, the molecular mechanisms underlying LT tolerance in cucumber remain largely unknown. Here, using a genome-wide association study (GWAS), we found a naturally occurring single nucleotide polymorphism (SNP) in the STAYGREEN (CsSGR) coding region at the gLTT5.1 locus associated with LT tolerance. Knockout mutants of CsSGR generated by clustered regularly interspaced palindromic repeats (CRISPR)/CRISPR-associated nuclease 9 exhibit enhanced LT tolerance, in particularly, increased chlorophyll (Chl) content and reduced reactive oxygen species (ROS) accumulation in response to LT. Moreover, the C-repeat Binding Factor 1 (CsCBF1) transcription factor can directly activate the expression of CsSGR. We demonstrate that the LT-sensitive haplotype CsSGRHapA, but not the LT-tolerant haplotype CsSGRHapG could interact with NON-YELLOW COLORING 1 (CsNYC1) to mediate Chl degradation. Geographic distribution of the CsSGR haplotypes indicated that the CsSGRHapG was selected in cucumber accessions from high latitudes, potentially contributing to LT tolerance during cucumber cold-adaptation in these regions. CsSGR mutants also showed enhanced tolerance to salinity, water deficit, and Pseudoperonospora cubensis, thus CsSGR is an elite target gene for breeding cucumber varieties with broad-spectrum stress tolerance. Collectively, our findings provide new insights into LT tolerance and will ultimately facilitate cucumber molecular breeding.
中文翻译：
STAYGREEN 的自然变异有助于黄瓜的低温耐受性
低温（LT）胁迫威胁全球黄瓜生产；然而，黄瓜 LT 耐受性的分子机制仍然很大程度上未知。在这里，利用全基因组关联研究 (GWAS)，我们在与 LT 耐受性相关的gLTT5.1位点的STAYGREEN ( CsSGR ) 编码区中发现了天然存在的单核苷酸多态性 (SNP)。由成簇规则间隔回文重复序列 (CRISPR)/CRISPR 相关核酸酶 9 产生的CsSGR敲除突变体表现出增强的 LT 耐受性，特别是响应 LT 的叶绿素 (Chl) 含量增加和活性氧 (ROS) 积累减少。此外，C重复结合因子1（CsCBF1）转录因子可以直接激活CsSGR的表达。我们证明LT敏感单倍型CsSGR HapA （而非LT耐受单倍型CsSGR HapG）可以与非黄色着色1（CsNYC1）相互作用以介导叶绿素降解。 CsSGR单倍型的地理分布表明， CsSGR HapG是在高纬度地区的黄瓜种质中选择的，可能有助于这些地区黄瓜冷适应过程中的 LT 耐受性。 CsSGR突变体还表现出对盐度、水分亏缺和古巴假霜霉的耐受性增强，因此CsSGR是选育广谱耐逆黄瓜品种的优良靶基因。总的来说，我们的研究结果为 LT 耐受性提供了新的见解，并将最终促进黄瓜分子育种。
更新日期：2023-10-09
中文翻译：
STAYGREEN 的自然变异有助于黄瓜的低温耐受性
低温（LT）胁迫威胁全球黄瓜生产；然而，黄瓜 LT 耐受性的分子机制仍然很大程度上未知。在这里，利用全基因组关联研究 (GWAS)，我们在与 LT 耐受性相关的gLTT5.1位点的STAYGREEN ( CsSGR ) 编码区中发现了天然存在的单核苷酸多态性 (SNP)。由成簇规则间隔回文重复序列 (CRISPR)/CRISPR 相关核酸酶 9 产生的CsSGR敲除突变体表现出增强的 LT 耐受性，特别是响应 LT 的叶绿素 (Chl) 含量增加和活性氧 (ROS) 积累减少。此外，C重复结合因子1（CsCBF1）转录因子可以直接激活CsSGR的表达。我们证明LT敏感单倍型CsSGR HapA （而非LT耐受单倍型CsSGR HapG）可以与非黄色着色1（CsNYC1）相互作用以介导叶绿素降解。 CsSGR单倍型的地理分布表明， CsSGR HapG是在高纬度地区的黄瓜种质中选择的，可能有助于这些地区黄瓜冷适应过程中的 LT 耐受性。 CsSGR突变体还表现出对盐度、水分亏缺和古巴假霜霉的耐受性增强，因此CsSGR是选育广谱耐逆黄瓜品种的优良靶基因。总的来说，我们的研究结果为 LT 耐受性提供了新的见解，并将最终促进黄瓜分子育种。
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