Biosynthesis of resveratrol by an endophytic Priestia megaterium PH3 via the phenylpropane pathway,Applied Microbiology and Biotechnology

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Biosynthesis of resveratrol by an endophytic Priestia megaterium PH3 via the phenylpropane pathway
Applied Microbiology and Biotechnology ( IF 3.9 ) Pub Date : 2023-10-06 , DOI: 10.1007/s00253-023-12768-x
Xiaohan Zhang _{1,

2} , Dong Zhang _{1,

2} , Yan Ding _{1,

2} , Zilong Li _{1,

2} , Chenchen Wang _{1,

2} , Shuhong Ye _{1,

2}

Affiliation

Abstract

Resveratrol (RES) is a secondary metabolite synthesized by plants in response to environmental stress and pathogen infection, which is of great significance for the industrial production of RES by fermentation culture. In this study, we aimed to explore the biosynthesis pathway of RES and its key enzymes in the Priestia megaterium PH3, which was isolated and screened from peanut fruit. Through Liquid Chromatography-Mass Spectrometry (LC-MS) analysis, we quantified the RES content and distribution in the culture medium and determined that Priestia megaterium PH3 mainly secreted RES extracellularly. Furthermore, the highest production of RES was observed in YPD, yielding an impressive 127.46 ± 6.11 μg/L. By optimizing the fermentation conditions, we achieved a remarkable RES yield of 946.82 ± 24.74 μg/L within just 2 days, which represents the highest reported yield for a natural isolate produced in such a short time frame. Our investigation revealed that the phenylpropane pathway is responsible for RES synthesis in this bacterium, with cinnamate 4-hydroxylase (C4H) identified as the main rate-limiting enzyme. Overall, our findings highlight the robust RES production capabilities of Priestia megaterium PH3, offering novel insights and potential applications for bacterial fermentation in RES production.

Key points

• RES synthesized by the bacterium was confirmed through the phenylpropane pathway.

• The key rate-limiting enzyme for biosynthesis-RES is C4H.

• RES reached 946.82 ± 24.74 μg/L after fermentation for 2 days.

Graphical Abstract

中文翻译：

内生Priestia megaterium PH3通过苯丙烷途径生物合成白藜芦醇

摘要

白藜芦醇（RES）是植物响应环境胁迫和病原菌感染而合成的次生代谢产物，对于发酵培养RES的工业化生产具有重要意义。本研究旨在探讨从花生果实中分离筛选的巨大Priestia megaterium PH3中RES及其关键酶的生物合成途径。通过液相色谱-质谱（LC-MS）分析，我们定量了培养基中RES的含量和分布，确定Priestia megaterium PH3主要在细胞外分泌RES。此外，在 YPD 中观察到 RES 产量最高，达到令人印象深刻的 127.46 ± 6.11 μg/L。通过优化发酵条件，我们在短短 2 天内实现了 946.82 ± 24.74 μg/L 的 RES 产量，这是在如此短的时间内生产的天然分离物的最高产量。我们的研究表明，苯丙烷途径负责该细菌中的 RES 合成，其中肉桂酸 4-羟化酶 (C4H) 被确定为主要限速酶。总体而言，我们的研究结果凸显了Priestia megaterium PH3强大的 RES 生产能力，为细菌发酵在 RES 生产中提供了新的见解和潜在应用。