<br>三(联吡啶)钌(II)衍生物纳米片聚集诱导增强电化学发光用于超灵敏检测人端粒酶RNA,Small

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三(联吡啶)钌(II)衍生物纳米片聚集诱导增强电化学发光用于超灵敏检测人端粒酶RNA
Small ( IF 13.0 ) Pub Date : 2023-09-29 , DOI: 10.1002/smll.202306291
Tingting Han _{1,

2} , Yu-Qian Geng ₂ , Min Zhang ₃ , Yue Cao ₂ , Jun-Jie Zhu _{2,

3}

Affiliation

传统的三(联吡啶)钌(II)配合物存在臭名昭著的聚集引起的猝灭效应，这极大地影响了其电化学发光(ECL)效率，从而阻碍了其在生物传感和临床诊断中的进一步应用。在这里，通过蛋白质辅助自组装策略合成了超薄四苯乙烯活性三（联吡啶）钌（II）衍生物纳米片（缩写为Ru-TPE NS），首次用于超灵敏ECL检测人端粒酶RNA（hTR）时间。合成的 Ru-TPE NS 表现出聚集诱导的增强 ECL 行为和优异的水分散性。令人惊讶的是，与有机溶液中分散的分子相比，Ru-TPE NS 的 ECL 效率提高了 106.5 倍。分子内运动的限制被证实是 ECL 显着增强的原因。因此，该ECL生物传感器基于Ru-TPE NSs作为高效ECL信标和催化发夹组件作为信号放大，对hTR表现出高灵敏度和优异的选择性，其检测限低至8.0 f m ，远远优于传统的ECL生物传感器。之前报道过的作品。这里，为早期临床诊断提供了一种有前景的分析方法，并展示了一种具有巨大应用前景的新型高效ECL发射器。

"点击查看英文标题和摘要"

Aggregation-Induced Enhanced Electrochemiluminescence from Tris(bipyridine)ruthenium(II) Derivative Nanosheets for the Ultrasensitive Detection of Human Telomerase RNA

The traditional tris(bipyridine)ruthenium(II) complex suffers from the notorious aggregation-caused quenching effect, which greatly compromises its electrochemiluminescence (ECL) efficiency, thus hindering further applications in biosensing and clinical diagnosis. Here, the ultrathin tetraphenylethylene-active tris(bipyridine)ruthenium(II) derivative nanosheets (abbreviated as Ru-TPE NSs) are synthesized through a protein-assisted self-assembly strategy for ultrasensitive ECL detection of human telomerase RNA (hTR) for the first time. The synthesized Ru-TPE NSs exhibit the aggregation-induced enhanced ECL behavior and excellent water-dispersion. Surprisingly, up to a 106.5-fold increase in the ECL efficiency of Ru-TPE NSs is demonstrated compared with the dispersed molecules in an organic solution. The restriction of intramolecular motions is confirmed to be responsible for the significant ECL enhancement. Therefore, this proposed ECL biosensor shows high sensitivity and excellent selectivity for hTR based on Ru-TPE NSs as efficient ECL beacons and the catalytic hairpin assembly as signal amplification, whose detection limit is as low as 8.0 fm, which is far superior to the previously reported works. Here, a promising analytical method is provided for early clinical diagnosis and a new type of efficient ECL emitters with great application prospects is represented.

更新日期：2023-09-29

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