下丘脑外侧区和基底前脑 PBN 末端的选择性光遗传学调节调节小鼠异氟醚麻醉苏醒,BMC Anesthesiology

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下丘脑外侧区和基底前脑 PBN 末端的选择性光遗传学调节调节小鼠异氟醚麻醉苏醒
BMC Anesthesiology ( IF 2.3 ) Pub Date : 2023-10-02 , DOI: 10.1186/s12871-023-02294-8
Kai Lu _{1,

2} , Zhenhuan Wang ₃ , Ning Bai ₁ , Ziyu Zhao ₁ , Xinrong Zhao ₁ , Yun He _{2,

4}

Affiliation

虽然全身麻醉的机制已被广泛研究，但潜在的神经回路尚未完全了解。臂旁核（PBN）在调节觉醒和促进全身麻醉唤醒方面发挥着至关重要的作用。然而，PBN 投影在全身麻醉过程中的具体作用仍不清楚。在这项研究中，我们将编码兴奋性或抑制性光遗传学探针的 AAV 相关病毒双侧注射到 PBN 中，并在 LH 或 BF 区域植入光纤。4 周后，我们在 1.5 vol% 异氟烷下通过光遗传学激活或抑制 PBN-LH 和 PBN-BF 通路。我们计算了麻醉诱导和苏醒所需的时间，同时使用脑电图记录监测突发抑制比的变化。我们的研究结果表明，PBN-LH 和 PBN-BF 投影的光遗传学激活在促进异氟醚吸入的皮质和行为出现方面发挥着重要作用，而不会显着影响诱导时间。相反，这些途径的光抑制延长了恢复时间，在诱导阶段没有观察到显着差异。总之，我们的结果表明，PBN-LH 和 PBN-BF 途径对于促进异氟烷全身麻醉的唤醒至关重要，但对诱导期没有明显影响。

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Selective optogenetic modulation of the PBN terminals in the lateral hypothalamic area and basal forebrain regulates emergence from isoflurane anesthesia in mice

While the mechanism of general anesthesia has been extensively studied, the underlying neural circuitry has yet to be fully understood. The parabrachial nucleus (PBN) plays a crucial role in modulating wakefulness and promoting arousal from general anesthesia. However, the specific role of PBN projections in the process of general anesthesia remains unclear. In this study, we bilaterally injected AAV-associated viruses encoding excitatory or inhibitory optogenetic probes into the PBN and implanted optical fibers in the LH or BF area. After four weeks, we optogenetically activated or inhibited the PBN-LH and PBN-BF pathways under 1.5 vol% isoflurane. We calculated the time it took for anesthesia induction and emergence, simultaneously monitoring changes in the burst-suppression ratio using electroencephalogram recording. Our findings indicate that optogenetic activation of the PBN-LH and PBN-BF projections plays a significant role in promoting both cortical and behavioral emergence from isoflurane inhalation, without significantly affecting the induction time. Conversely, photoinhibition of these pathways prolonged the recovery time, with no notable difference observed during the induction phase. In summary, our results demonstrate that the PBN-LH and PBN-BF pathways are crucial for promoting arousal from isoflurane general anesthesia, but do not have a pronounced impact on the induction phase.

更新日期：2023-10-02

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