Syngas (CO, CO₂, H₂) was effectively bioconverted into lipids in a two-stage process. In the first stage, C₁-gases were bioconverted into acetic acid by the acetogenic species Clostridium aceticum through the Wood-Ljungdahl metabolic pathway in a stirred tank bioreactor, reaching a maximum acetic acid concentration of 11.5 g/L, with a production rate of 0.05 g/L·h. Throughout this experiment, samples were extracted at different periods, i.e., different concentrations, to be used in the second stage, aiming at the production of lipids from acetic acid. The yeast Rhodosporidium toruloides, inoculated in the acetogenic medium, was able to efficiently accumulate lipids from acetic acid generated in the first stage. The best results, in terms of lipid content, dry biomass, biomass yield (Y_(X/S)) and lipid yield (Y_(L/S)) were 39.5% g/g dry cell weight, 3 g/L, 0.35 and 0.107, respectively. In terms of abundance, the lipid profile followed the order: C18:1 > C16:0 > C18:2 > C18:0 > Others. Experiments were also performed to determine the toxicity exerted by high concentrations of acetic acid on R. toruloides, resulting in inhibition at initial acid concentrations around 18 g/L leading to a higher lag phase and being lethal to the yeast at initial acetic acid concentrations around 22 g/L and above. This research paves the way for a novel method of growing oleaginous yeasts to produce sustainable biofuels from syngas or C₁-pollutant gases.

合成气（CO、CO ₂、H ₂）通过两阶段过程有效地生物转化为脂质。第一阶段，C ₁ -气体在搅拌釜生物反应器中通过Wood-Ljungdahl代谢途径被产乙酸菌醋酸梭菌生物转化为乙酸，最高乙酸浓度为11.5 g/L，产率为0.05克/升·小时。在整个实验中，在不同时期（即不同浓度）提取样品，用于第二阶段，目的是从乙酸生产脂质。接种在产乙酸培养基中的酵母红冬孢酵母能够有效地从第一阶段产生的乙酸中积累脂质。就脂质含量、干生物量、生物量产量 (Y _(X/S) ) 和脂质产量 (Y _(L/S) ) 而言，最佳结果为 39.5% g/g 细胞干重、3 g/L、0.35和0.107，分别。就丰度而言，脂质谱的顺序为：C18:1 > C16:0 > C18:2 > C18:0 > 其他。还进行了实验以确定高浓度乙酸对圆红酵母产生的毒性，在初始酸浓度约为 18 g/L 时产生抑制，导致更高的滞后期，并在初始乙酸浓度约为 18 g/L 时对酵母致命。 22克/升及以上。这项研究为培育产油酵母以从合成气或 C ₁污染物气体生产可持续生物燃料的新方法铺平了道路。