A Mexican isolate of the nematophagous fungus Arthrobotrys musiformis was obtained from a soil sample from the Chapultepec ecological reserve zone, in Cuernavaca, Morelos, Mexico. This isolate demonstrated an important predatory activity (74.9%) against the parasitic nematode Haemonchus contortus (L3) and its fungal liquid culture filtrates (LCF) grown in two media showed the following highest nematocidal activities (NA): In Czapek-DoxBroth (CzDoxB) 80.66% and potato-dextrose broth (PDB) 49.84%. Additionally, two major compounds derived from carboxylic acids and two derivates from alkane group were identified by GC–MS. These compounds have been associated to many biological activities. On the other hand, the protein profile analysis by SDS-electrophoresis followed by a zymogram revealed a 10 kDa protein with protease activity. This study provides important information for future experiments focused to explore the potential use of this protein as well as the identified bioactive compounds presents in the LCF as potential candidates against sheep haemonchosis.

从墨西哥莫雷洛斯州库埃纳瓦卡查普尔特佩克生态保护区的土壤样本中分离出墨西哥线虫类真菌节孢菌。该分离株表现出对寄生线虫捻转血矛线虫(L3)的重要捕食活性 (74.9%) ，其在两种培养基中生长的真菌液体培养滤液 (LCF) 显示出以下最高的杀线虫活性 (NA)： 在 Czapek-DoxBroth (CzDoxB) 中80.66% 和马铃薯葡萄糖肉汤 (PDB) 49.84%。此外，通过 GC-MS 鉴定了两种源自羧酸的主要化合物和两种源自烷烃基团的衍生物。这些化合物与许多生物活性有关。另一方面，通过 SDS 电泳进行蛋白质谱分析，然后进行酶谱分析，结果显示具有蛋白酶活性的 10 kDa 蛋白质。这项研究为未来的实验提供了重要信息，重点是探索这种蛋白质的潜在用途以及 LCF 中已鉴定的生物活性化合物作为抗羊血吸虫病的潜在候选者。