Biosurfactants have found widespread use across multiple industrial fields, including medicine, food, cosmetics, detergents, pulp, and paper, as well as the degradation of oil and fat. The culture broth of Aureobasidium pullulans A11231-1-58 isolated from flowers of Chrysanthemum boreale Makino exhibited potent surfactant activity. Surfactant activity-guided fractionation led to the isolation of three new biosurfactants, pullusurfactins A‒C (1‒3). Their chemical structures were established through the use of spectroscopic techniques, predominantly 1D and 2D NMR, in conjunction with mass measurements. We evaluated the surface tension activities of isolated compounds. At 1.0 mg l⁻¹, these compounds showed high degrees of surfactant activity (31.15 dyne/cm, 33.75 dyne/cm, and 33.83 dyne/cm, respectively).

生物表面活性剂已广泛应用于多个工业领域，包括医药、食品、化妆品、洗涤剂、纸浆和纸张以及油脂的降解。从菊花牧野花中分离出的出芽短梗霉A11231-1-58 的培养液表现出有效的表面活性剂活性。表面活性剂活性引导的分馏分离出三种新的生物表面活性剂：普鲁表面活性剂 A-C ( 1-3 )。它们的化学结构是通过使用光谱技术（主要是一维和二维核磁共振）并结合质量测量来确定的。我们评估了分离化合物的表面张力活性。在1.0mg l ^-1时，这些化合物显示出高程度的表面活性剂活性（分别为31.15达因/厘米、33.75达因/厘米和33.83达因/厘米）。