Four undescribed cycloartane-type triterpenoids (1−4) and seven undescribed steroids (6−12), along with five known analogues (5 and 13−16), were isolated from the leaves of Trichilia connaroides. Their structures were identified based on the NMR data and HRESIMS, and the absolute configurations were determined through single-crystal X-ray diffraction analysis, Mosher's method, and ECD calculations. The multidrug resistance (MDR) reversal activities of all the isolates were assessed, and compounds 10 and 11 showed significant activities to reverse the MDR of MCF-7/DOX cells with IC₅₀ values of 2.90 and 3.76 μM, respectively. These bioactive compounds may bring fresh insights into the research and development of MDR reversal agents.

从Trichilia connaroides的叶子中分离出了四种未描述的环阿麻烷型三萜类化合物 ( 1 – 4 ) 和七种未描述的类固醇 ( 6 – 12 )，以及五种已知的类似物（5和13 – 16 ） 。根据NMR数据和HRESIMS鉴定了它们的结构，并通过单晶X射线衍射分析、Mosher法和ECD计算确定了绝对构型。评估了所有分离株的多药耐药（MDR）逆转活性，化合物10和11显示出显着的逆转MCF-7/DOX细胞MDR的活性，IC ₅₀值分别为2.90和3.76 μM。这些生物活性化合物可能为MDR逆转剂的研发带来新的见解。