<br>药物输送中的动态光散射和透射电子显微镜：正确表征纳米颗粒和解释结果的路线图,Materials Horizons

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药物输送中的动态光散射和透射电子显微镜：正确表征纳米颗粒和解释结果的路线图
Materials Horizons ( IF 12.2 ) Pub Date : 2023-09-26 , DOI: 10.1039/d3mh00717k
Sergey K Filippov _{1,

2} , Ramil Khusnutdinov ₃ , Anastasiia Murmiliuk ₄ , Wali Inam ₂ , Lucia Ya Zakharova ₅ , Hongbo Zhang _{2,

6} , Vitaliy V Khutoryanskiy ₁

Affiliation

在这篇焦点文章中，我们对透射电子显微镜 (TEM) 和动态光散射 (DLS) 作为研究纳米颗粒尺寸的两种常用方法进行了仔细分析，重点关注其在制药和药物输送中的应用。对纳米颗粒尺寸和形状的控制是许多生物医学系统的关键因素之一。粒径将极大地影响它们通过生物膜的渗透。例如，增强的渗透和保留效应需要非常窄的纳米颗粒尺寸范围（50-200 nm），即使与这些值的微小偏差也会严重影响药物纳米载体向肿瘤的输送。然而，令人惊讶的是，大量药剂学和药物输送领域的研究论文报告了通过两种最流行的实验技术（TEM 和 DLS）测量的纳米颗粒尺寸的显着差异。在某些情况下，据报道这种差异为 200-300%，这就提出了哪种尺寸测量结果更值得信赖的问题。在这篇焦点文章中，我们主要关注导致 TEM 和 DLS 结果之间常规观察到的不匹配的物理方面。其中一些因素（例如浓度和角度依赖性）通常被低估和误解。我们令人信服地证明，正确使用实验程序并对使用两种方法生成的结果进行彻底分析可以完全消除 DLS 和 TEM 数据不匹配，或者使结果彼此更加接近。此外，我们还为药物输送和药物研究人员提供了清晰的路线图，以进行可靠的 DLS 测量。

"点击查看英文标题和摘要"

Dynamic light scattering and transmission electron microscopy in drug delivery: a roadmap for correct characterization of nanoparticles and interpretation of results

In this focus article, we provide a scrutinizing analysis of transmission electron microscopy (TEM) and dynamic light scattering (DLS) as the two common methods to study the sizes of nanoparticles with focus on the application in pharmaceutics and drug delivery. Control over the size and shape of nanoparticles is one of the key factors for many biomedical systems. Particle size will substantially affect their permeation through biological membranes. For example, an enhanced permeation and retention effect requires a very narrow range of sizes of nanoparticles (50–200 nm) and even a minor deviation from these values will substantially affect the delivery of drug nanocarriers to the tumour. However, amazingly a great number of research papers in pharmaceutics and drug delivery report a striking difference in nanoparticle size measured by the two most popular experimental techniques (TEM and DLS). In some cases, this difference was reported to be 200–300%, raising the question of which size measurement result is more trustworthy. In this focus article, we primarily focus on the physical aspects that are responsible for the routinely observed mismatch between TEM and DLS results. Some of these factors such as concentration and angle dependencies are commonly underestimated and misinterpreted. We convincingly show that correctly used experimental procedures and a thorough analysis of results generated using both methods can eliminate the DLS and TEM data mismatch completely or will make the results much closer to each other. Also, we provide a clear roadmap for drug delivery and pharmaceutical researchers to conduct reliable DLS measurements.

更新日期：2023-09-26

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