Sulfur dioxide (SO₂) derivatives are extensively utilized as both a preservative for foods and an active gaseous signal molecule in various physiological and pathological processes, but their excessive intake would bring harmful effects on human health; so, the determination of SO₂ derivatives is of great importance. Herein, we developed a ratiometric fluorescent probe named 2-(2′-hydroxyphenyl)benzothiazole-3-ethyl-1,1,2-trimethyl-1H-benzo[e]indolium (HBT-EMBI) by introducing a hemicyanine unit of EMBI to an HBT group for the detection of SO₂ derivatives via an excited-state intramolecular proton transfer (ESIPT) and intramolecular charge transfer (ICT) effects. The probe displays some advantages, such as a colorimetric change from purple to colorless, a ratiometric fluorescence with zero cross-talk, and a remarkably large emission shift (Δλ = 164 nm) under a single-wavelength excitation. Accordingly, the probe HBT-EMBI has been successfully employed for the colorimetric and ratiometric determination of SO₂ derivatives in real food samples and the quantitative visualization of SO₂ derivative variations in HepG2 cells.

中文翻译：

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开发用于检测食品和活细胞中 SO2 衍生物的零串扰比例荧光探针

二氧化硫(SO ₂ )衍生物广泛用作食品防腐剂和各种生理病理过程中的活性气体信号分子，但过量摄入会对人体健康带来有害影响。_{因此，SO 2}衍生物的测定具有重要意义。在此，我们通过引入半花青单元开发了一种比率荧光探针，命名为2-(2′-羟苯基)苯并噻唑-3-乙基-1,1,2-三甲基-1H-苯并[e]吲哚( HBT - EMBI) EMBI 到 HBT 基团，用于通过激发态分子内质子转移 (ESIPT) 和分子内电荷转移 (ICT) 效应检测 SO _{2衍生物。}该探针具有一些优点，例如比色从紫色变为无色、零串扰的比率荧光以及单波长激发下显着大的发射位移（Δλ = 164 nm）。_{因此，探针HBT-EMBI已成功用于实际食品样品中SO 2}衍生物的比色和比率测定以及HepG2细胞中SO ₂衍生物变化的定量可视化。