Fibrous scar is one of the major factors that hinder functional recovery in patients with spinal cord injury (SCI). Studies have shown that the laminin α1 peptide chain ile-les-val-ala-Val (IKVAV) promoted axonal growth and motor function recovery in rats after SCI. However, whether IKVAV could ameliorate SCI via reducing the formation of fibrous scar was not clear. A SCI model was constructed by transecting the rat spinal cord with a scalpel and implanting poly (N-propan-2-ylprop-2-enamide) (PNIPAM)-b-poly (AC-PEG-COOH) (PNPP) or PNIPAM-b-poly (AC-PEG-IKVAV) (PNPP-IKVAV) hydrogel. 14 days later hematoxylin-eosin staining and immunohistochemical staining were used to assess the effect of PNPP-IKVAV on scar formation. The effect of PNPP-IKVAV on endoplasmic reticulum (ER) stress was investigated by immunohistochemical staining. NIH-3T3 cells were used for in vitro scratching experiments and a transforming growth factor 1 (TGF-β1) activation model was constructed to assess the role of PNPP-IKVAV. In this study, PNPP-IKVAV inhibited fibroblast migration and suppressed TGF-β1 activation and ER stress (ERS) to reduce the extracellular matrix secretion by fibroblasts.

纤维性疤痕是阻碍脊髓损伤（SCI）患者功能恢复的主要因素之一。研究表明，层粘连蛋白α1肽链ile-les-val-ala-Val（IKVAV）促进SCI后大鼠轴突生长和运动功能恢复。然而，IKVAV 是否​​可以通过减少纤维疤痕的形成来改善 SCI 尚不清楚。用手术刀横切大鼠脊髓，植入聚(N-丙-2-基丙-2-烯酰胺)(PNIPAM)-b-聚(AC-PEG-COOH)(PNPP)或PNIPAM-构建SCI模型。 b-聚（AC-PEG-IKVAV）（PNPP-IKVAV）水凝胶。14天后，使用苏木精-伊红染色和免疫组织化学染色来评估PNPP-IKVAV对疤痕形成的影响。通过免疫组织化学染色研究 PNPP-IKVAV 对内质网 (ER) 应激的影响。使用NIH-3T3细胞进行体外划痕实验，构建转化生长因子1（TGF-β1）激活模型来评估PNPP-IKVAV的作用。在这项研究中，PNPP-IKVAV 抑制成纤维细胞迁移并抑制 TGF-β1 激活和 ER 应激 (ERS)，从而减少成纤维细胞的细胞外基质分泌。