As a significant kind of reactive oxygen species (ROS), peroxynitrite (ONOO⁻) plays an indispensable role in many physiological and pathological processes. This study aimed to synthesize a novel dihydro-benzo[4,5]imidazo[1,2-c]quinazoline-based probe 1 for detecting ONOO⁻. In 99.5% H₂O solution, probe 1 displayed a distinct aggregation-induced ratiometric emission (AIRE), and would selectively respond toward ONOO⁻ via a ratiometric fluorescent signal, along with a short response time (<30 s) and ultra-sensitivity (LOD = 17.6 nM). Moreover, the probe was applied for monitoring the concentration fluctuations of ONOO⁻ in HeLa cells and zebrafish.

中文翻译：

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一种基于二氢苯并[4,5]咪唑[1,2-c]喹唑啉的探针，具有聚集诱导的比率发射，用于活细胞和斑马鱼中过氧亚硝酸盐的比率荧光检测

^{过氧亚硝酸盐（ONOO -} ）作为一类重要的活性氧（ROS），在许多生理和病理过程中发挥着不可或缺的作用。本研究旨在合成一种新型二氢苯并[4,5]咪唑并[1,2- c ]喹唑啉基探针1，用于检测ONOO ^-。在 99.5% H ₂ O 溶液中，探针1显示出独特的聚集诱导比率发射 (AIRE)，并且通过比率荧光信号选择性地响应 ONOO ^， 同时响应时间短（<30 秒）和超灵敏（LOD = 17.6 nM）。此外，该探针还用于监测HeLa细胞和斑马鱼中ONOO ^-的浓度波动。